用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物
用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物
Invitrogen™

用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物

使用用于检测吞噬作用的 pH 敏感性 pHrodo Red Zymosan BioParticles 偶联物,实现更快的染色,得到更准确的结果,且无需洗涤步骤或淬灭染料。此染料-生物颗粒偶联物相较于其他染料-生物颗粒偶联物可得到更快、更精确的结果,可降低敏感实验中的信号变异性并改善时序了解更多信息
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货号数量
P35364Promo Image5 x 1 mg
货号 P35364
价格(CNY)
4,233.00
飞享价
Ends: 31-Dec-2025
5,739.00
共减 1,506.00 (26%)
Each
有货
添加至购物车
数量:
5 x 1 mg
物流信息
常规产品: 发货后, 北上广深及省会城市通常为 1-2天,二线城市通常 2-3天,三线以及偏远地区通常 4天,周末以及节假日顺延。
空运受限产品: 发货后, 北上广深及省会城市通常为 2-4天,二线城市 通常3-5天,三线以及偏远地区通常 6-10天,周末以及节假日顺延。
现货中心: 此类产品将由您身边的现货中心极速配送。
价格(CNY)
4,233.00
飞享价
Ends: 31-Dec-2025
5,739.00
共减 1,506.00 (26%)
Each
添加至购物车
使用用于检测吞噬作用的 pH 敏感性 pHrodo Red Zymosan BioParticles 偶联物,实现更快的染色,得到更准确的结果,且无需洗涤步骤或淬灭染料。此染料-生物颗粒偶联物相较于其他染料-生物颗粒偶联物可得到更快、更精确的结果,可降低敏感实验中的信号变异性并改善时序。pHrodo Red 染料偶联物在细胞外无荧光,但在吞噬体中呈红色强荧光,可专门用于检测细胞内吞噬和内吞作用。pHrodo Red Zymosan BioParticles 偶联物也可与多种蓝色、绿色和远红色染料报告基因(如 GFP、Fuo-4、钙黄绿素、NucBlue、CellEvent Caspase 3/7 green、Mitosox Green 和 Mitotracker Deep Red 等)配合使用进行多通路检测。

pHrodo Red 染料的荧光随 pH 值从中性到酸性降低而显著增强,使其成为研究吞噬作用以及该作用受药物和/或环境因素调节情况的出色工具。它在细胞外无荧光,无需洗涤步骤和淬灭染料。

即用型 pHrodo Red Zymosan BioParticles 偶联物用于成像或流式应用,或 pHrodo Red SE(活化琥珀酰亚胺酯)用于标记您选择的微生物或蛋白。

此外,pHrodo 染料可提供绿色,并可作为大肠杆菌、酵母聚糖、金黄色葡萄球菌和葡聚糖 10,000 MW 的偶联物提供。

仅供科研使用。不可用于人或动物的治疗或诊断。
规格
描述用于检测吞噬作用的 pHrodo™ Red Zymosan BioParticles™ 偶联物
检测方法荧光
染料类型pH 值敏感的染料
激发/发射560/585
数量5 x 1 mg
运输条件室温
种属酿酒酵母
颜色红色
适用于(应用)细胞分析
适用于(设备)Attune™ 声波聚焦流式细胞仪, 共聚焦显微镜, Floid™ 细胞成像系统, 荧光显微镜, 高内涵分析仪, 流式细胞仪
产品线pHrodo™
产品类型染料
Unit SizeEach
内容与储存
在 –20°C 干燥条件下避光储存。

phrodo-red-5-internalization
live-cell-tracking-with-phrodo-dyes

图表

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文件和下载

证书

批号Certificate TypeDateCatalog Number(s)
3090248Certificate of Analysis2025年4月03日P35364
2836761Certificate of Analysis2024年2月14日P35364
2604405Certificate of Analysis2023年6月16日P35364
2454200Certificate of Analysis2022年5月03日P35364
2364486Certificate of Analysis2021年5月26日P35364
显示 5 条结果, 在上面搜索特定证书

安全数据表

常见问题解答 (FAQ)

Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (11)

引用和文献
Abstract
Inflammatory dysregulation of blood monocytes in Parkinson's disease patients.
Authors:Grozdanov V, Bliederhaeuser C, Ruf WP, Roth V, Fundel-Clemens K, Zondler L, Brenner D, Martin-Villalba A, Hengerer B, Kassubek J, Ludolph AC, Weishaupt JH, Danzer KM
Journal:
PubMed ID:25284487
Despite extensive effort on studying inflammatory processes in the CNS of Parkinson's disease (PD) patients, implications of peripheral monocytes are still poorly understood. Here, we set out to obtain a comprehensive picture of circulating myeloid cells in PD patients. We applied a human primary monocyte culture system and flow cytometry-based ... More
GPR37 regulates macrophage phagocytosis and resolution of inflammatory pain.
Authors:
Journal:J Clin Invest
PubMed ID:30010619
Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing.
Authors:
Journal:Neuron
PubMed ID:30606613
A common haplotype lowers PU.1 expression in myeloid cells and delays onset of Alzheimer's disease.
Authors:
Journal:Nat Neurosci
PubMed ID:28628103
The Alzheimer's disease-associated protective Plcγ2-P522R variant promotes immune functions.
Authors:
Journal:Mol Neurodegener
PubMed ID:32917267
11 total citations

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