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Invitrogen™
Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.
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| Catalog Number | Quantity | Assay | Quantitation Range |
|---|---|---|---|
| Q33230 | 100 assays | 1X dsDNA, high sensitivity | 0.1 to 120 ng |
| Q33231 | 500 assays | 1X dsDNA, high sensitivity | 0.1 to 120 ng |
| Q33266 | 500 assays | 1X dsDNA, broad range | 4 to 4000 ng |
| Q33265 | 100 assays | 1X dsDNA, broad range | 4 to 4000 ng |
Catalog number Q33230
Price (USD)
147.65
Online Exclusive
154.00Save 6.35 (4%)
Each
-
Quantity:
100 assays
Assay:
1X dsDNA, high sensitivity
Quantitation Range:
0.1 to 120 ng
Price (USD)
147.65
Online Exclusive
154.00Save 6.35 (4%)
Each
Experience easy, accurate, and precise quantification of dsDNA with Qubit 1X dsDNA HS (High Sensitivity) and Qubit 1X dsDNA BR (Broad Range) Assay Kits. The 1X formulation provides a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometers. These assays are highly selective for dsDNA over ssDNA, RNA, protein, and free nucleotides. Contaminants, such as salts, solvents, or detergents are well-tolerated.
Qubit 1X dsDNA HS and BR Assay Kits, designed for use with Qubit Fluorometers, are highly selective for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA), RNA, protein, and free nucleotides. All kits provide a ready-to-use working solution and DNA standards. To perform the assay, simply dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a Qubit Fluorometer.
Qubit 1X dsDNA HS Assay Kit
The Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 5 pg/μL to 120 ng/μL, providing a detection range of 0.1−120 ng.
Qubit 1X dsDNA BR Assay Kit
The Qubit dsDNA BR (Broad-Range) Assay Kit, designed for use with Qubit 4 or Qubit Flex Fluorometers, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 4,000 ng/μL, providing a detection range of 4-4,000 ng.
Notes
• Qubit 1X dsDNA HS can be used with Qubit 2, Qubit 3, Qubit 4, and Qubit Flex Fluorometers
• Qubit 1X dsDNA BR are designed for use with Qubit 4 and Qubit Flex Fluorometers
• Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200 μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer
Qubit 1X dsDNA HS Assay Kit
The Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 5 pg/μL to 120 ng/μL, providing a detection range of 0.1−120 ng.
Qubit 1X dsDNA BR Assay Kit
The Qubit dsDNA BR (Broad-Range) Assay Kit, designed for use with Qubit 4 or Qubit Flex Fluorometers, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 4,000 ng/μL, providing a detection range of 4-4,000 ng.
Notes
• Qubit 1X dsDNA HS can be used with Qubit 2, Qubit 3, Qubit 4, and Qubit Flex Fluorometers
• Qubit 1X dsDNA BR are designed for use with Qubit 4 and Qubit Flex Fluorometers
• Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200 μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay1X dsDNA, high sensitivity
For Use With (Equipment)Qubit 2.0, 3, 4, and Flex Fluorometers
Green FeaturesFewer resources used, Less waste
No. of Reactions100 Reactions
Product LineQubit
Quantitation Range0.1 to 120 ng
Quantity100 assays
Shipping ConditionWet Ice
Unit SizeEach
Enhance your Qubit Fluorometer with the latest firmware
New Qubit 4 and Qubit Flex firmware features include importing sample IDs, renaming files before exporting, a countdown timer, and on-board troubleshooting guidance. Get the most out of your Qubit assays with these powerful new tools.
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Lot #Certificate TypeDateCatalog Number(s)
3148412Certificate of AnalysisMay 18, 2025Q33231
3148354Certificate of AnalysisApr 10, 2025Q33230
3006788Certificate of AnalysisApr 09, 2025Q33231
3021911Certificate of AnalysisApr 06, 2025Q33231
3080140Certificate of AnalysisMar 30, 2025Q33230
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Product Information
Frequently asked questions (FAQs)
The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Cat. Q33230, Q33231, Q33265, Q33266) are stable for 6 months from the date of receipt when stored as directed.
For more information on product storage, please refer to the user guides:
Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
The Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Cat. Nos. Q33230, Q33231, Q33265, Q33366) are newer and have a simplified workflow compared to the original Qubit dsDNA Quantification Assay Kits (Cat. Nos. Q32850, Q32851, Q32853, Q32854). The original Qubit kits contain separate dyes and buffer components that must be mixed together to make a working solution, in which the dye degrades after a few hours. With the newer Qubit 1X dsDNA Assay kits, the dye is premixed with a buffer that keeps the dye stable long-term and can be added directly to DNA samples.
You can find more information about the Qubit 1X dsDNA assay, by clicking on the Technical Note provided below:
Qubit 1X dsDNA assays: simplified workflow and improved performance
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
The Qubit 1X dsDNA High Sensitivity (HS) (Cat. Nos. Q33230, Q33231) and Broad Range (BR) Assay Kits (Cat. Nos. Q33265, Q33266) have different storage conditions.
For Qubit 1X dsDNA HS Assay Kits (Cat. Nos. Q33230, Q33231), store all components at 2 - 8 degrees C.
For Qubit 1X dsDNA BR Assay Kits (Cat. Nos. Q33265, Q33266), store the working solution at 18 - 28 degrees C. Store the standards at 2 - 8 degrees C.
The assay kits are stable for at least 6 months from the date of receipt.
For more information, please refer to these user guides:
Qubit 1X dsDNA HS Assay Kit and Qubit 1X dsDNA BR Assay Kit
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
Citations & References (10)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Plasmodium-specific atypical memory B cells are short-lived activated B cells.
Journal:Elife
PubMed ID:30387712
'A subset of atypical memory B cells accumulates in malaria and several infections, autoimmune disorders and aging in both humans and mice. It has been suggested these cells are exhausted long-lived memory B cells, and their accumulation may contribute to poor acquisition of long-lasting immunity to certain chronic infections, such
Prevalence of Germline Mutations Associated With Cancer Risk in Patients With Intraductal Papillary Mucinous Neoplasms.
Journal:Gastroenterology
PubMed ID:30716324
'Many patients with pancreatic adenocarcinoma carry germline mutations associated with increased risk of cancer. It is not clear whether patients with intraductal papillary mucinous neoplasms (IPMNs), which are precursors to some pancreatic cancers, also carry these mutations. We assessed the prevalence of germline mutations associated with cancer risk in patients
Co-digestion of organic and mineral wastes for enhanced biogas production: Reactor performance and evolution of microbial community and function.
Journal:Waste Manag
PubMed ID:31109531
'Mineral wastes (MWs) from municipal solid waste incineration plants and construction demolition sites are rich in minerals, heavy metals and have acid neutralising capacity. This renders such MWs a promising source of bulk and trace elements to enhance and stabilize biogas production in anaerobic processes. However, finding a MW with
Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation.
Journal:Int J Mol Sci
PubMed ID:31337116
'Regulatory T cells (T'
Genomic Epidemiology of SARS-CoV-2 in Guangdong Province, China.
Journal:Cell
PubMed ID:32359424
'Coronavirus disease 2019 (COVID-19) is caused by SARS-CoV-2 infection and was first reported in central China in December 2019. Extensive molecular surveillance in Guangdong, China''s most populous province, during early 2020 resulted in 1,388 reported RNA-positive cases from 1.6 million tests. In order to understand the molecular epidemiology and genetic
10 total citations