Ninhydrin Reagent is used to detect sodium hippurate hydrolysis by beta-hemolytic streptococci, G. vaginalis, and C. jejuni.Hippuric acid is hydrolyzed by the enzyme hippuricase to glycine and benzoic acid. Ninhydrin evokes a five-step reaction to form hydrindantin (a reduced form of ninhydrin), carbon dioxide, and ammonia. A condensation reaction happens with hydrindantin, ammonia and residual ninhydrin to produce a final purple colored complex3.Ready for use: no further preparation is required.Easy to interpret:Positive test: Blue to purple color development within 30 minutes.Negative test: Light grey color development or no color change within 30 minutes.Ninhydrin Reagent contains ninhydrin (CAS 485-47-2), acetone (CAS 67-64-1), butanol (CAS 71-36-3) supplied in 25 mL bottles. This product is for in vitro diagnostic use and should be used by properly trained individuals. This reagent have been tested using quality control procedure and found to be acceptable.For use with Hippurate Disk, REF R2108Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.