Streptavidin, Alexa Fluor™ 488 Conjugate
Streptavidin, Alexa Fluor™ 488 Conjugate
Invitrogen™

Streptavidin, Alexa Fluor™ 488 Conjugate

Alexa Fluor™ 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin hasRead more
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Catalog NumberQuantityForm
S112231 mgSolid
S323540.5 mLLiquid
Catalog number S11223
Price (USD)
338.00
Each
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Quantity:
1 mg
Form:
Solid
Price (USD)
338.00
Each
Add to cart
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Alexa Fluor™ 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:
Alexa Fluor™ 488 streptavidin conjugate has Ex/Em maxima of ∼ (495/519)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit (Cat. No. E21390) to minimize this interference.

See User Manual for solubility instructions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission495/519 nm
Label or DyeAlexa Fluor
Product TypeStreptavidin Conjugate (fluorescent)
Quantity1 mg
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 488
FormSolid
Product LineAlexa Fluor™
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C) and protect from light.
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Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Figures

Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3174403Certificate of AnalysisJun 30, 2025S11223
2833447Certificate of AnalysisFeb 19, 2024S11223
2720376Certificate of AnalysisOct 19, 2023S32354
2622396Certificate of AnalysisFeb 28, 2023S11223
43773ACertificate of AnalysisFeb 17, 2023S11223
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Citations & References (93)

Citations & References
Abstract
SHAP potentiates the CD44-mediated leukocyte adhesion to the hyaluronan substratum.
Authors:Zhuo L,Kanamori A,Kannagi R,Itano N,Wu J,Hamaguchi M,Ishiguro N,Kimata K
Journal:The Journal of biological chemistry
PubMed ID:16702221
A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes.
Authors:Beeton C, Wulff H, Singh S, Botsko S, Crossley G, Gutman GA, Cahalan MD, Pennington M, Chandy KG
Journal:J Biol Chem
PubMed ID:12511563
T lymphocytes with unusually high expression of the voltage-gated Kv1.3 channel (Kv1.3(high) cells) have been implicated in the pathogenesis of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. We have developed a fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1.3, for detection of Kv1.3(high) cells ... More
Inhibition of platelet-derived growth factor-BB-induced receptor activation and fibroblast migration by hyaluronan activation of CD44.
Authors:Li L, Heldin CH, Heldin P
Journal:J Biol Chem
PubMed ID:16809345
'The extracellular matrix molecule hyaluronan was found to suppress platelet-derived growth factor (PDGF) beta-receptor activation and PDGF-BB-induced migration of primary human dermal fibroblasts. The suppressive effect of hyaluronan was neutralized by a monoclonal antibody that specifically inhibits hyaluronan binding to its receptor CD44. Moreover, co-immunoprecipitation experiments showed that the PDGF ... More
PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.
Authors:Golestaneh N, Fan J, Zelenka P, Chepelinsky AB,
Journal:Mol Vis
PubMed ID:18523655
'PURPOSE: To investigate the functional significance of MIP/AQP0 phosphorylation at serine(235). METHODS: MIP/AQP0 expression and cellular localization was studied in rat lens epithelia explants induced to differentiate by FGF-2. MIP wild type (WT) and MIP (S235A) mutant expression plasmids were constructed and transiently expressed in RK13 cells. Subcellular localization of ... More
An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.
Authors:Trinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,
Journal:Blood
PubMed ID:19828693
'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More
93 total citations

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