HBSS (10X), calcium, magnesium, phenol red
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Gibco™

HBSS (10X), calcium, magnesium, phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
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Catalog NumberQuantity
14060073
also known as 14060-073
10 x 500 mL
14060040500 mL
Catalog number 14060073
also known as 14060-073
Price (ARS)
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Quantity:
10 x 500 mL
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This HBSS is modified as follows:

  • With: Calcium, Magnesium, Glucose, Phenol Red
  • Without: Sodium Bicarbonate

The complete formulation is available.

Concentrated forms of Gibco™ HBSS require pH adjustment and filtration at the time of preparation (see protocol for details).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveCalcium and Magnesium
ClassificationAnimal Origin-Free
Dilution10X
Product LineGibco™
Product TypeHanks' Balanced Salt Solution (HBSS)
Quantity10 x 500 mL
Solution TypeHank's Balanced Salt
Osmolality2500 to 3000 mOsm/kg
pH5.5 to 6.0
Phenol Red IndicatorPhenol Red
SterilitySterile-filtered
FormLiquid
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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Frequently asked questions (FAQs)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it true that phenol red has estrogenic properties?

Phenol red in tissue culture is a weak estrogen. Implications concerning study of estrogen-responsive cells in culture are discussed in Proc Natl Acad Sci U S A 83:2496 (1986).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3168530Certificate of AnalysisApr 18, 202514060040
2368957Certificate of AnalysisJan 29, 202514060040
2715538Certificate of AnalysisJan 29, 202514060040
2783410Certificate of AnalysisJan 29, 202514060040
2798094Certificate of AnalysisJan 28, 202514060040
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