HBSS (10X), no calcium, no magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Concentrated forms of Gibco™ HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).