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Thermo Scientific™
PageRuler™ Prestained NIR Protein Ladder
Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250 kDa) that are blue-stained Read more
Catalog number 26635
Price (USD)/ 2 x 250 µL
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Quantity:
2 x 250 mL
Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250 kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Compare and view all other protein standards and ladders ›
Product features
• Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers
• Reference band—55 kDa band has greater intensity for easy orientation
Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on NIR fluorescent SDS-polyacrylamide gels and western blots
Compare and view all other protein standards and ladders ›
Product features
• Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers
• Reference band—55 kDa band has greater intensity for easy orientation
Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on NIR fluorescent SDS-polyacrylamide gels and western blots
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, NIR Fluorescence (700 nm)
Number of Markers10
Product TypeProtein Ladder
Ready to LoadYes
Size Range11 to 250 kDa
Stain Type1 color: Blue
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa
Product LinePageRuler™
Quantity2 x 250 mL
Shipping ConditionApproved for shipment on Wet or Dry Ice
System TypeWestern Blotting, SDS-PAGE
Unit Size2 x 250 µL
Contents & Storage
Contents: two vials of 250 μL each
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C