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TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
Actual product may vary
Applied Biosystems™

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR inRead more
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Catalog NumberNo. of Reactions
4366072500 Reactions
43678465000 Reactions
4352042250 Reactions
43660731250 Reactions
43641032500 Reactions
Catalog number 4366072
Price (USD)
-
No. of Reactions:
500 Reactions
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TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-μL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Convenience
Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)2 X
For Use With (Equipment)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
FormatTube
No. of Reactions500 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq DNA Polymerase
Product LineAmpErase™, TaqMan™
Product TypeFast Universal PCR Master Mix
Quantity500 reactions
Sample TypeDNA (Genomic), cDNA
Sufficient For500 Reactions
Volume20 μL
Concentration2X
Detection MethodPrimer-probe
For Use With (Application)Gene Expression, Genotyping, miRNA analysis
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 2 packs of 2 tubes of 1.25 mL each sufficient for 500 20 μL PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
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Frequently asked questions (FAQs)

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

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