The new Ion Xpress™ RNA-Seq Barcode 1-16 Kit provides a set of 16 unique barcode primers for sequencing RNA on the Ion Torrent PGM™ semiconductor sequencer. When used in combination with the Ion Total RNA-Seq Kit v2 (Cat. No. 4475936), the Ion Xpress™ RNA-Seq Barcode 1-16 Kit enables users to pool up to 16 fragment libraries prior to template preparation and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for small and whole transcriptome RNA.

Features and Benefits:

• Efficient multiplexing-enables multiplexing of up to 16 fragment libraries on a single sequencing chip
• Both sequence- and flow-optimized for equal representation of all barcodes in a pool
• Multiplexing decreases the cost and the handling requirements of template preparation and sequencing
• Robust error correction built in by design for added confidence in sample identification

Efficient Multiplexing with Barcode Primers Enables Higher Throughput Sample Sequencing

Multiplexing with barcoded libraries leads to more cost-effective runs by permitting up to 16 samples per run, significantly decreasing the cost and handling requirements of template preparation and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency

Barcode primers contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and necessitate only 20 bases of barcode and adaptor sequencing, which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In By Design

Built-in error correction significantly minimizes the possibility of incorrect sample assignment.

The Ion Xpress™ RNA-Seq Barcode 01-16 Kit is designed to create 10 multiplexed libraries per barcode or a total of 160 multiplexed libraries.