SuperSignal™ Enhanced Molecular Weight Protein Ladder
Thermo Scientific™
SuperSignal™ Enhanced Molecular Weight Protein Ladder
Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins withRead more
Have Questions?
Catalog number 84786
Price (USD)/ 250 µL
-
Quantity:
250 μL
Request bulk or custom format
Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
Two versions of the MW marker are offered. This formulation should be used in applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody. The SuperSignal Molecular Weight Protein ladder formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodUser defined detection system
Number of Markers8
Product TypeProtein Ladder
Ready to LoadYes
Size Range20 to 150 kDa
Stain TypeUnstained
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels