Axiom™ BloodGenomiX Array Kit

Developed through collaboration with international blood service groups and hematology experts, the Applied Biosystems Axiom BloodGenomiX Array, along with the specialized BloodGenomiX Reporter Software, offers a comprehensive solution for precise blood genotyping in blood research. The innovative Axiom BloodGenomiX Array enables labs to detect a wide range of extended and rare blood group systems, as well as tissue (HLA) and platelet (HPA) types, in a single, high-throughput assay.

The Axiom BloodGenomiX Array Kit includes all necessary array plates, reagents, and consumables to process 768 samples (two 384-well plates) using the Axiom Propel XPRES 2x384HT workflow.

The Axiom BloodGenomiX Array includes over 2,500 markers that can be used to type common (Rh, Kell, Duffy) and rare blood groups to perform research in immunohematology, alloimmunization, maternal–fetal incompatibility, and hemoglobinopathies. The blood typing content on the array includes genetic research markers for anemia, bleeding disorders, and thalassemia. The array supports detailed copy number calling in the RHD gene and genotyping of variants in the highly homologous RHD and RHCE genes in the Rh blood group system. The array also combines the use of direct genotyping with advanced imputation methods over the extended MHC region, allowing accurate HLA typing from SNP genotype data.

Content coverage

• Comprehensive coverage with nearly 20,000 probe-variant pairs, meticulously designed for research of genetic variations in blood group, HLA, and HPA class of genes relevant for transfusion therapy
• Superior coverage for European, African, admixed American, East Asian, and South Asian populations
• Human erythrocyte antigens (HEA) variants in 38 relevant blood group systems, providing valuable screening information for finding rare or uncommon donors for clinical research
• Human platelet antigens (HPA) and human neutrophil antigens (HNA), targeting the most important antigens identified by global blood experts
• Human leukocyte antigen (HLA) typing, covering HLA class I genes relevant for research insights related transfusion therapy
• Interrogation of hemoglobin S and C carriers in donors for research purposes
• Extensive coverage of iron regulation, specifically addressing hereditary hemochromatosis and providing research insights related to iron-deficiency anemia in donors
• Examination of blood product characteristics, including restless leg syndrome, helping enable a holistic understanding of donor health profiles
• Evaluation of variants potentially associated with product quality related to potassium leakage, hemolysis, and storage stability

BloodGenomiX Reporter Software

Axiom BloodGenomiX genotype analysis has been integrated seamlessly into the Automated Axiom Analysis software. BloodGenomiX Reporter Software transforms genotyping data output from the GeneTitan instrument into HEA, HNA, and HPA types. For each tested sample, the BloodGenomiX Reporter software determines HEA, HNA, and HPA types by directly typing relevant DNA variants associated with antigen expression. The embedded HLA software then employs imputation on the genotyping results to predict HLA Class I antigen types against a globally maintained database of known reference haplotypes. The final output flat file from BloodGenomiX Reporter has been formatted with direct feedback from blood blanks to allow for easier integration into electronic records used in blood service organizations.

The ability to generate phenotype reports using the Automated Axiom Analysis Suite is acquired by purchasing the desired number of reports (see Cat. Nos. 00.1064 (1 report) and 00.1065 (768 reports)).

Axiom myDesign Custom Array Services

For those looking for deeper scientific insights or to include specific SNPs relevant to their disease research area or population-specific variants of interest, Axiom myDesign Custom Array Services are available for the creation of a custom array.