VitroEase™ Methylamine Vanadate Negative Stain

Thermo Scientific VitroEase Methylamine Vanadate Negative Stain is an easy, ready-to-use negative stain for electron microscopy (EM) analysis.

Features of VitroEase Methylamine Vanadate Negative Stain include:
• Provided as a stable, ready-to-use 2% stain solution
• Easy to use with straight-forward staining protocol
• Uniform, electron-dense stain for EM
• Dries without crystallization, ideal for EM grids

VitroEase Methylamine Vanadate Negative Stain is a ready-to-use stain with excellent spreading properties at a near-neutral pH of 8. It produces a light, uniform negative stain for high-resolution fine structure visualization and does not denature protein samples. This stain is also stable and non-volatile in the electron beam, making it more resistant than uranyl acetate negative stain.

References:
1. Franzetti, B.; Schoehn, G.; Hernandez, J. F.; Jaquinod, M.; Ruigrok, R. W.; and Zaccai, G.: Tetrahedral aminopeptidase: a novel large protease complex from archaea. EMBO J., 21, 2132-2138 (2002).
2. Gregori, L., Hainfeld, J. F., Simon, M. N., and Goldgaber, D. Binding of amyloid beta protein to the 20S proteasome. J. Biol. Chem., 272, 58-62 (1997).
3. Hainfeld, J.F., Safer, D., Wall, J.S., Simon, M., Lin, B., and Powell, R. D. Methylamine vanadate (NanoVan) negative stain. In Proc. 52nd Ann. Mtg. Micros. Soc. Amer.; Bailey, G.W. and Garratt-Reed, A.J. (Eds.), San Francisco Press, San Francisco. 132-133, (1994).
4. Zagursky, R. J.; Ooi, P.; Jones, K. F.; Fiske, M. J.; Smith, R. P., and Green, B. A. Identification of a Haemophilus influenzae 5'-nucleotidase protein: cloning of the nucA gene and immunogenicity and characterization of the NucA protein. Infect. Immun., 68, 2525-34 (2000).