CTS™ HiFi Cas9 Protein and CTS™ Xenon Genome Editing Buffer Kit
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CTS™ HiFi Cas9 Protein and CTS™ Xenon Genome Editing Buffer Kit
Gibco™

CTS™ HiFi Cas9 Protein and CTS™ Xenon Genome Editing Buffer Kit

This kit includes CTS HiFi Cas9 Protein and CTS Xenon Genome Editing Buffer (also available separately). CTS HiFi Cas9 ProteinRead more
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Catalog NumberQuantity
A542852.5 mg (Cas9), 1 x 100 mL (Buffer)
A542875 mg (Cas9), 2 x 100 mL (Buffer)
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Catalog number A54285
Price (USD)/ 2.5 mg
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Quantity:
2.5 mg (Cas9), 1 x 100 mL (Buffer)

This kit includes CTS HiFi Cas9 Protein and CTS Xenon Genome Editing Buffer (also available separately).

CTS HiFi Cas9 Protein
Gibco CTS HiFi Cas9 Protein is our GMP-manufactured high-fidelity CRISPR-Cas9 protein, engineered to deliver reduced off-target effects while maintaining high on-target activity. This protein is designed and manufactured in compliance with the principles of GMP and to meet requirements of the standards for Ancillary Materials for Cell, Gene, and Tissue-Based Products. It is provided in a high-concentration and large-scale format for process development and clinical research applications.

Features of CTS HiFi Cas9 Protein include:
Exceptional off-target profile—over 70% reduction in off-target effects in primary immune cells, compared to CTS TrueCut Cas9 (wild type) at tested loci
Maintain on-target activity—achieve comparable on-target activity to CTS TrueCut Cas9 (wild type) in primary immune cells at tested loci
Stringent manufacturing process—manufactured to meet the standards for Ancillary Materials for Cell, Gene, and Tissue-Based Products including USP , Ph.Eur. 5.2.12, and ISO 20399 following the principles of 21 CFR Part 820 in an FDA-registered manufacturing site
Extensive safety testing—includes sterility, endotoxin, mycoplasma, residual nucleic acid, and protein testing
Traceability documentation—COA, COO, Drug Master files, and Regulatory Support files* are available

CTS HiFi Cas9 Protein is a Streptococcus pyogenes high-fidelity Cas9 protein, produced by recombinant DNA technology in E. coli for genome editing with CRISPR technology. Cas9 protein forms a very stable RNP complex with the guide RNA (gRNA) component of the CRISPR-Cas9 system. Incorporation of NLS aids delivery to the nucleus, increasing the editing efficiency and on-target cleavage.

If you would like to evaluate our HiFi Cas9 protein or conduct small-scale research experiments, we recommend using our research-grade TrueCut HiFi Cas9 Protein.

CTS Xenon Genome Editing Buffer
Gibco CTS Xenon Genome Editing Buffer is designed specifically for use with the Xenon Electroporation System. It has been designed to improve performance with gene editing-specific payloads (e.g., CRISPR Cas9) for knock-out- or knock-in-based applications in a variety human primary cells.


*Country-specific Regulatory Support File process will be initiated upon request and will require additional documentation time.

For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research. Caution: Not intended for direct administration into humans or animals.
Specifications
Concentration10 mg/mL
Quantity2.5 mg (Cas9), 1 x 100 mL (Buffer)
Shelf Life18 months from date of manufacture
Product TypeCas9 Protein and Buffer kit
Shipping ConditionDry Ice
Expression SystemE. coli
FormLiquid
Research CategoryGenome Editing
Manufacturing QualityISO 13485
Unit Size2.5 mg
Contents & Storage
Contains 2.5 mg CTS HiFi Cas9 Protein and 100 mL CTS Xenon Genome Editing Buffer. Store at –20°C.