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Catalog Number | Unit Size | Price (USD) | |
---|---|---|---|
F175S | 100 reactions | Contact Us › | |
F175L | 500 reactions | Contact Us › |
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.
This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions and from various species.
Highlights
Applications
The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
For optimal results start by accurately calculating your Tm with our Tm calculator.