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Zitierungen und Referenzen (7)
Gibco™
B-27™ Supplement (50x), serumfrei
B-27-Supplement ist das häufigste zitierte Supplement für die neuronale Zellkultur. Das optimierte serumfreie Supplement ermöglicht das Wachstum embryonaler, postnataler undWeitere Informationen
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| Katalognummer | Menge |
|---|---|
| 17504044 | 10 mL |
| 17504001 | 100 mL |
Katalognummer 17504044
Preis (EUR)
119,00
Each
-
Menge:
10 mL
Preis (EUR)
119,00
Each
B-27-Supplement ist das häufigste zitierte Supplement für die neuronale Zellkultur. Das optimierte serumfreie Supplement ermöglicht das Wachstum embryonaler, postnataler und erwachsener hippocampaler Neuronen und anderer Neuronen des ZNS in niedriger oder hoher Dichte sowie mit kurz- oder langfristiger Lebensfähigkeit. B-27 Supplement ist als 50x Flüssigkeit erhältlich und für die Verwendung mit Neurobasal Medium oder Neurobasal-A Medium für neuronale Zellkulturen ohne erforderliche Astrozyten-Nährschicht konzipiert.
B-27 Supplement eignet sich für die Verwendung in den meisten Anwendungen mit Nervenzellen. Für andere Anwendungen bieten wir alternative Formulierungen von B-27 Supplement und Neurobasal Medien.
Für etablierte Protokolle, die B-27 Supplement verwenden, rufen Sie unser Gibco Handbuch für Neurobiologieprotokolle auf.
Sehen Sie sich auf unserer Gibco CellCite Veröffentlichungen zu Gibco Neurobiologieprodukten an.
B-27 Supplement eignet sich für die Verwendung in den meisten Anwendungen mit Nervenzellen. Für andere Anwendungen bieten wir alternative Formulierungen von B-27 Supplement und Neurobasal Medien.
Für etablierte Protokolle, die B-27 Supplement verwenden, rufen Sie unser Gibco Handbuch für Neurobiologieprotokolle auf.
Sehen Sie sich auf unserer Gibco CellCite Veröffentlichungen zu Gibco Neurobiologieprodukten an.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ZelltypNervenzellen
Konzentration50 X
KulturtypSäugetierzellkultur
ProduktlinieB-27™
Menge10 mL
Serumspiegelserumfrei
Haltbarkeit12 Monate
VersandbedingungTrockeneis
Quelleserumfrei
Getestet aufEndotoxin, Leistungsfähigkeit, Sterilität, Mykoplasma
Klassifikationserumfrei
FormFlüssig
ProdukttypZellkultur-Supplement
SterilitätSteril
pH6 bis 8
Unit SizeEach
Inhalt und Lagerung
Bei -5 bis -20 °C lagern und vor Licht schützen.
Medienformulierungen
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Abbildungen
Primary rat Hippocampus neurons cultured in B-27 Supplement
Rat cortical neurons cultured in Neurobasal Medium with B-27 Supplement
Primary rat cortex neurons cultured in B-27 Supplement
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Zertifikate
Nach Chargennummer oder Teil-Chargennummer suchen
Chargen-Nr.Certificate TypeDateCatalog Number(s)
2954071Certificate of Analysis22. Juni 202517504044, 17504001
2954081Certificate of Analysis22. Juni 202517504044, 17504001
3135631Certificate of Analysis21. Juni 202517504044, 17504001
2954065Certificate of Analysis03. Mai 202517504044, 17504001
3011455Certificate of Analysis23. Apr. 202517504044, 17504001
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Sicherheitsdatenblätter
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Product Information
Häufig gestellte Fragen (FAQ)
Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .
We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.
For more information on Neurobasal media, search "Neurobasal" from our website home page.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Zitierungen und Referenzen (7)
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Zitierungen und Referenzen
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Journal:Experimental cell research
PubMed ID:38499143
In vitro generation of human pluripotent stem cell derived lung organoids.
Journal:
PubMed ID:25803487
'Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Journal:Nature
PubMed ID:21151107
Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have therapeutic efficacy in
Axonal Transport Enables Neuron-to-Neuron Propagation of Human Coronavirus OC43.
Journal:J Virol
PubMed ID:29925652
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs are not always confined to the upper respiratory tract and can invade the central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies in humans are
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Journal:Cell Stem Cell
PubMed ID:32579880
SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from
7 total citations