Der Pierce Modifizierte Lowry-Protein-Assay ist die stabile Form eines traditionellen, zweikomponentigen, Folinphenol- und kupferbasierten Reagenziensystems zur Messung der Gesamtproteinkonzentration imWeitere Informationen
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Katalognummer
Menge
23240
530 ml
Katalognummer 23240
Preis (EUR)
224,65
Online Exclusive
249,00
Ersparnis 24,35 (10%)
Each
-
Zum Warenkorb hinzufügen
Menge:
530 ml
Recurring order eligible. Learn more »
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
224,65
Online Exclusive
249,00
Ersparnis 24,35 (10%)
Each
Zum Warenkorb hinzufügen
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Der Pierce Modifizierte Lowry-Protein-Assay ist die stabile Form eines traditionellen, zweikomponentigen, Folinphenol- und kupferbasierten Reagenziensystems zur Messung der Gesamtproteinkonzentration im Vergleich zu einem Proteinstandard.
Merkmale des modifizierten Lowry-Protein-Assays: • Beliebte Methode — in der Proteinforschung häufig zitiert wird • Kolorimetrisch — Messung mit einem Standard-Spektralphotometer oder Plattenlesegerät bei 750 nm • Stabil — verwendet ein modifiziertes Kupfersulfat-Tartrat-Reagenz, das bei Raumtemperatur stabil ist • Assay-Bereich — zeigt gute Linearität im Bereich von 1 bis 1500 µg/ml (getestet mit BSA-Protein)
Das modifizierte Lowry Proteinassay-Kit kombiniert eine stabilisierte Formulierung der ursprünglichen Lowry Reagenzien mit dem essentiellen Folin-Ciocalteu Phenol-Reagenz in einem kompletten Kit zur genauen Bestimmung der Proteinkonzentration in einer Vielzahl von Probentypen. Obwohl neuere Proteinassay-Methoden eine höhere Geschwindigkeit und Praktikabilität bieten, bleibt die Lowry-Methode für viele Anwendungen eine beliebte, genaue und nützliche Option.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
AssayLowry-Assay
Zur Verwendung mit (Anwendung)Lösungsbasierter Nachweis, Extinktion
Zur Verwendung mit (Geräte)Spektralphotometer, Mikrotiterplatten-Lesegerät
ProduktliniePierce™
ProdukttypAssay zur Proteinquantifizierung
Menge530 ml
SpezifitätNicht zielspezifisch
Ausreichend für480 Assays in Röhrchen oder 2400 Assays auf Mikrotiterplatten
NachweisverfahrenKolorimetrisch
Unit SizeEach
Inhalt und Lagerung
Ausreichend für: 480 Röhrchen-Assays oder 2400 Mikrotiterplatten-Assays
• Proteinassay-Reagenz für den modifizierten Lowry-Test, 480 ml • 2N Folin-Ciocalteu Phenol-Reagenz, 50 ml • Albumin Standard-Ampullen, 2 mg/ml, 10 x 1 ml
Bei 4 °C lagern.
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Häufig gestellte Fragen (FAQ)
Which Thermo Scientific protein assay is the best or the most reliable?
The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. Nos. 23236, A55866) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).
I purchased the Pierce Modified Lowry Protein Assay Kit (Cat. No. 23240). The label on the bottle states that it should be stored at 4 degrees C, whereas the box that the kit arrives in states to store at 2-8 degrees C. Which is correct?
The entire kit (3 components) can be stored at 4°C. The storage of individual components is listed below:
The Lowry Protein Assay Reagent should be stored at 4°C.
The 2N Folin-Ciocalteu Reagent has been tested for one year at both 4°C and room temperature, and shows no difference in performance with both storage conditions.
The Albumin Standard Ampules can be stored at 4°C or room temperature. Stability testing for one year at 4°C and at room temperature has also been performed for the Albumin Standards.
Can you provide the shelf-life for the Pierce Modified Lowry Protein Assay Kit?
The Pierce Modified Lowry Protein Assay Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
I assayed two protein samples, each containing a different mixture of proteins of same concentration and observed very different color responses in the assay. What is the cause?
Each of the commonly used total protein assay methods exhibits some degree of varying response toward different proteins. These differences relate to amino acid sequence, pI, structure and the presence of certain side chains or prosthetic groups that can dramatically alter the protein’s color response. Most protein assay methods use BSA or immunoglobulin (IgG) as the standard against which the concentration of protein in the sample is determined. However, if great accuracy is required, prepare the standard curve from a pure sample of the target protein.
My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?
You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.