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Gibco™
CTS™ Virale Produktionszellen
CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTSWeitere Informationen
| Katalognummer | Menge |
|---|---|
| A53956 | 1.5 mL |
Katalognummer A53956
Preis (EUR)Kontaktieren Sie uns
-
Menge:
1.5 mL
CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTS LV-MAX lentiviral production system to produce maximal lentiviral titers. The cells allow high density, serum-free suspension culture with minimal cell clumping in CTS LV-MAX Production Medium. They are specifically validated for lentiviral vector production for the purpose of cell and gene therapy applications, but may be suitable for the production of other viral vectors where the absence of SV40 large T antigen in HEK293 cells is desired. These cGMP-banked cells have gone through a documented validation process to ensure that they can be transfected with multiple plasmids and that viable viral particles can be produced.
Features of the CTS Viral Production Cells include:
- Fully-documented cGMP-banked cell line (HEK293F-derived) with detailed cell line lineage history
- Extensive quality testing based on the US FDA and European Medicines Agency (EMA) guidances for therapeutic use
- Flexible licensing options without royalties
Please visit www.thermofisher.com/celllicenserequest for more information and access.
Für die Verwendung in der Forschung oder die Herstellung von zell-, gen- oder gewebebasierten Produkten. ACHTUNG: Nicht für Anwendungen an Menschen oder Tieren geeignet.
Specifications
Zur Verwendung mit (Anwendung)Cell & Gene Therapy Research, Development, and Manufacturing
FormatCryopreserved vial
Fertigungsqualität, Herstellungsqualität21 CFR Parts 210 and 211
MedienempfehlungGibco™ CTS™ Viral Production Medium
Anzahl ZellenSee Certificate of Analysis
ProdukttypViral Production Cell Line
Menge1.5 mL
Haltbarkeit10 years from date of manufacture
VersandbedingungDry ice or vapor phase liquid nitrogen
Getestet aufLV Production
Zelllinie293F
ZelltypNiere (Embroyonisch)
Culture TypeSuspensions-Zellkultur
Unit SizeEach
Inhalt und Lagerung
1 x 107 cells in a 2-mL vial. Store in vapor phase liquid nitrogen.
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Zertifikate
Nach Chargennummer oder Teil-Chargennummer suchen
Chargen-Nr.Certificate TypeDateCatalog Number(s)
271319-MCB1Certificate of Analysis02. Juli 2025A53956
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Sicherheitsdatenblätter
SDBHäufig gestellte Fragen (FAQ)
No, CTS Viral Production Cells are HEK 293F suspension cells that have been adapted to CTS LV-MAX Production Medium. They do not express the SV40 large T antigen.
CTS Viral Production Cells can be frozen directly in cryogenic medium consisting of 90% CTS LV-MAX Production Medium plus 10% DMSO. Here is the protocol:
- Bank cells at passage 3 when cell density reaches ˜3.0-4.5 x 10E6 viable cells/mL at >95% viability.
- Centrifuge the cells at 100 x g (low speed) for 5 mins to pellet. Discard the spent medium.
- Prepare freezing medium: e.g., 10 mL of freezing medium is 9 mL of CTS LV-MAX Production Medium + 1 mL DMSO; mix well and sterilize by 0.2 µm filtration.
- Resuspend the cell pellet in the prepared freezing medium.
- Dilute the cells to a final density of 10 x10E6 viable cells/mL and aliquot 1 mL per cryovial.
- Freeze the cells at -80 degrees C for 1 day.
- Transfer frozen vials to liquid nitrogen for long-term storage.
CTS Viral Production Cells 2.0 are specifically designed to work with CTS AAV MAX products to provide maximal adeno-associated virus (AAV) titers and optimal performance. CTS Viral Production Cells can be used but we don't recommend doing this as it will reduce system performance.