1x1 image pixel for data collection
Probenecid, wasserlöslich
Probenecid, wasserlöslich
Invitrogen™

Probenecid, wasserlöslich

Probenecid wird häufig verwendet, um organische Anionentransporter in der Zellmembran zu hemmen. Solche Transporter können Farbstoffe und Indikatoren extrudieren undWeitere Informationen
Have Questions?
KatalognummerMenge
P3640010 x 77 mg
Katalognummer P36400
Preis (EUR)
450,65
Online Exclusive
479,00
Ersparnis 28,35 (6%)
Each
Vorrätig
Zum Warenkorb hinzufügen
Menge:
10 x 77 mg
Recurring order eligible. Learn more »
Preis (EUR)
450,65
Online Exclusive
479,00
Ersparnis 28,35 (6%)
Each
Zum Warenkorb hinzufügen
Ask our AI about this Product
Probenecid wird häufig verwendet, um organische Anionentransporter in der Zellmembran zu hemmen. Solche Transporter können Farbstoffe und Indikatoren extrudieren und so zu einer schlechten Beladung oder einem hohen Hintergrundsignal in Assays beitragen, die auf der Retention der Farbstoffe oder Indikatoren innerhalb von Zellen basieren. Die Verwendung von Probenecid zum Blockieren des Efflux intrazellulärer Farbstoffe wurde zunächst von Di Virgilio et al. (1990) beschrieben, und es wurde mit einer breiten Palette anionischer Farbstoffe und Konjugate verwendet. Die häufig verwendete freie Säureform von Probenecid ist schwer aufzulösen, sodass 1 M NaOH es in Lösung bringen muss. Unser wasserlösliches Probenecid (P36400) löst sich schnell im Assay-Puffer auf und macht den Umgang mit ätzendem NaOH überflüssig. Das wasserlösliche Probenecid ist in den Fluo-4 NW Calcium-Assay-Kits (F36205, F36206) enthalten und auch separat erhältlich.

Erfahren Sie mehr über Ionen-Indikatoren wie Calcium-, Kalium-, pH- und Membranpotential-Indikatoren ›

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Menge10 x 77 mg
Empfohlene LagerungBei Raumtemperatur lagern
VersandbedingungRaumtemperatur
Physikalische FormFest
ProdukttypReagenz für die Zellmarkierung
Unit SizeEach
four-plex-image-COS7-live-cell zoomed
PG2624-PJT8477-COL022341-Media-Promo-Pod-Graphic-Global-F-278x123
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Häufig gestellte Fragen (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Customers who viewed this item also viewed



Dokumente und Downloads

Zertifikate

Chargen-Nr.Certificate TypeDateCatalog Number(s)
3143083Certificate of Analysis17. Apr. 2025P36400
2935434Certificate of Analysis06. Juli 2024P36400
2810926Certificate of Analysis16. Feb. 2024P36400
2743073Certificate of Analysis29. Sept. 2023P36400
2600149Certificate of Analysis31. Mai 2023P36400
5 Ergebnisse angezeigt, oben nach einem bestimmten Zertifikat suchen

Sicherheitsdatenblätter

Product Information

Zitierungen und Referenzen (21)

Zitierungen und Referenzen
Abstract
The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons.
Authors:Geng Y, Hoke A, Delpire E,
Journal:J Biol Chem
PubMed ID:19307180
'NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information. In a recent study, it was shown that peripheral nerve injury results in increased NKCC1 activity, not due to an increase in cotransporter expression, but to increased phosphorylation of the cotransporter (Pieraut, S., ... More
A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.
Authors:Clementi EA, Marks LR, Duffey ME, Hakansson AP,
Journal:J Biol Chem
PubMed ID:22700972
'To cause colonization or infection, most bacteria grow in biofilms where differentiation and death of subpopulations is critical for optimal survival of the whole population. However, little is known about initiation of bacterial death under physiological conditions. Membrane depolarization has been suggested, but never shown to be involved, due to ... More
Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers.
Authors:Di Virgilio F, Steinberg TH, Silverstein SC
Journal:Cell Calcium
PubMed ID:2191781
Fura-2 is widely used to measure the concentration of cytosolic free calcium, but in many cells the dye does not remain localized within the cytoplasmic matrix. In these cells, Fura-2 is sequestered within intracellular organelles, secreted into the extracellular medium, or both. We have found that, in mouse peritoneal macrophages, ... More
Genetic divergence of rotavirus nonstructural protein 4 results in distinct serogroup-specific viroporin activity and intracellular punctate structure morphologies.
Authors:Hyser JM, Utama B, Crawford SE, Estes MK,
Journal:J Virol
PubMed ID:22357281
Nonstructural protein 4 (NSP4) viroporin activity is critical for the replication and assembly of serogroup A rotavirus (RVA); however, the dramatic primary sequence divergence of NSP4s across serogroups raises the possibility that viroporin activity is not a common feature among RVs. We tested for NSP4 viroporin activity from divergent strains, ... More
Cell-based potassium ion channel screening using the FluxOR assay.
Authors:Beacham DW, Blackmer T, O' Grady M, Hanson GT,
Journal:J Biomol Screen
PubMed ID:20208034
FluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the FluxOR Potassium Ion Channel Assay is based on the activation of a novel fluorescent dye. This indicator reports channel activity with a ... More
21 total citations

Geben Sie die Katalognummer, den Namen oder Link frei.

1x1 image pixel for data collection