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High-Capacity RNA-to-cDNA™ Kit
High-Capacity RNA-to-cDNA™ Kit
Applied Biosystems™

High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression MasterRead more
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Catalog NumberNo. of Reactions
438740650 Reactions
4388950500 Reactions
Catalog number 4387406
Price (EUR)
206,05
Special offer
Online exclusive
Ends: 15-Aug-2025
317,00
Save 110,95 (35%)
Each
In stock
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No. of Reactions:
50 Reactions
Price (EUR)
206,05
Special offer
Online exclusive
Ends: 15-Aug-2025
317,00
Save 110,95 (35%)
Each
Add to cart

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatKit
No. of Reactions50 Reactions
Optimal Reaction Temperature37°C
Quantity50 reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Reverse TranscriptaseMultiScribe
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
Size (Final Product)Up to 7 kb
Starting MaterialRNA
TechniqueReverse Transcription
For Use With (Application)Real Time PCR (qPCR), RT-PCR
Reaction Speed30 to 60 min.
Unit SizeEach
Contents & Storage

• 20X Enzyme mix (1 x 50 μL)
• 2X RT Buffer Mix (1 x 500 μL)

Store at –15 to –25°C.
Buffer Mix may be stored at 2–8°C for up to 6 months.

Frequently asked questions (FAQs)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can I order the RNase Inhibitor from the High-Capacity cDNA Reverse Transcription Kit separately?

Yes. You can order the RNase Inhibitor separately as Cat. No. N8080119.

What type of primers are in the High-Capacity RNA-to-cDNA Kit?

The High Capacity RNA-to-cDNA Kit includes a 2X RT buffer mix, which is composed of dNTPs, random octamers, and oligo(dT)16.

When using the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919), I get high Ct values. What can I do?

It could be due to the following reasons:

  • Insufficient cDNA template is present. Use 10 to 100 ng of cDNA template per 50 µL reaction.
  • Quality of cDNA template is poor. Quantify the amount of cDNA template and test the cDNA template for the presence of PCR inhibitors.
  • Sample degradation. Prepare fresh cDNA, then repeat the experiment.
  • Reduced number of PCR cycles in the thermal cycling protocol. Increase the number of PCR cycles to the default setting of 40.
  • Primer-dimer formation and residual polymerase activity. Prepare the reaction mixes and reaction plate on ice. To ensure optimal results, run the reaction plate as soon as possible after completing the reaction setup.


Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

When using the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919), the Multicomponent signal for ROX is not linear. What could cause this?

This could be due to the following reasons:

  • Pure dye component’s spectra are incorrect. Rerun pure dye spectra.
  • Incorrect dye components have been chosen. Choose correct dyes for data analysis.


Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

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