Human Plasma-Like Medium (HPLM)

Gibco Human Plasma-like Medium (HPLM) is designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma.

• Physiologically relevant—designed to resemble the natural cellular environment found in the body; formulated with over than 60 polar metabolites and plasma-like salt concentrations
• Peer reviewed—extensive publication of research that used HPLM
• Easy to use—direct replacement for conventional media when supplemented with FBS

The widely used classic synthetic cell culture media, including MEM, DMEM, RPMI 1640, and DMEM/F-12 contain glucose, amino acids, vitamins, and salts at concentrations that often do not reflect those found in human plasma. These media also lack additional plasma components needed to mimic the metabolic profile of human plasma. When studying cancer and other diseases, results with more physiological relevance will enable researchers to improve their understanding of human function and illness.

The HPLM solution

HPLM contains more than 60 polar metabolites such as amino acids, nucleic acids, sugars, and small organic acids at concentrations found in human plasma. The salt concentrations in HPLM mimic the salt concentrations found in human plasma. In resembling the natural cellular environment found in the body, HPLM provides researchers with the ability to study the impact of physiologically relevant cell media on their specific applications.

When supplemented with fetal bovine serum (FBS), HPLM is capable of supporting cell growth and viability comparable to those of conventional basal media supplemented with FBS. For most cell lines, adaptation is not required to transition from conventional medium to HPLM.

The complete HPLM formulation is available (hyperlink to technical-resource page with complete HPLM formulation).

cGMP manufacturing and quality system

HPLM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.