The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0. The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, which are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium.
Features of the AAV-MAX Transfection Kit include: • Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified) • AAV-MAX transfection reagent—cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures • AAV-MAX booster—chemically defined component that works with transfection reagent to achieve high transfection efficiency • AAV-MAX enhancer—chemically defined component that enhances the production of AAV particles • Robust transfection protocols are adaptable to large scale transfection
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Line
HEK293
Culture Type
Suspension Cell Culture
Description
Serum-free, animal origin-free, chemically defined
Cell Type
Established Cell Lines
Format
Bottle
Sample Type
Plasmid DNA
Transfection Technique
Lipid-based Transfection
Expression System
Mammalian
For Use With (Equipment)
AAV-MAX System
For Use With (Application)
AAV Production
Includes
10 mL Gibco AAV-MAX Enhancer, 6 mL Gibco AAV-MAX Transfection Reagent, 3 mL Gibco AAV-MAX Supplement
Product Line
AAV-MAX
Product Type
AAV production transfection Kit
Quantity
1 kit for 1 L of production
Reagent Type
Transfection kit
Shelf Life
12 months from date of manufacture
Shipping Condition
Wet Ice
Sterility
Sterile
Unit Size
For 1 L of culture
Contents & Storage
• 6 mL AAV-MAX Trasfection Reagent • 3 mL AAV-MAX Transfection Booster • 10 mL AAV-MAX Enhancer
Reagents should be stored at 2–8°C. Do not freeze.