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AAV-MAX Helper-Free AAV Production System Kit
The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based onRead more
| Catalog Number | Quantity |
|---|---|
| A51217 | Sufficient for 1 L of Culture |
Catalog number A51217
Price (EUR)
1.762,00
Each
Estimated availability date 07-Jul-2025
Quantity:
Sufficient for 1 L of Culture
Price (EUR)
1.762,00
Each
The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols. Features of the AAV-MAX Helper-Free AAV Production System include:
• Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection The components included in the AAV-MAX System Kit are:
• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement All components are also available for purchase separately.
• Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection The components included in the AAV-MAX System Kit are:
• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement All components are also available for purchase separately.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineViral Production Cells 2.0
Cell TypeKidney (Embryonic)
For Use With (Application)AAV Production
Product LineAAV-MAX
Product TypeAAV Production System
QuantitySufficient for 1 L of Culture
Shelf Life12 months from date of manufacture
Culture TypeSuspension Cell Culture
SterilitySterile
Unit SizeEach
Contents & Storage
• 1 vial Viral Production Cells 2.0
• 1 L Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement
Storage conditions vary
• 1 L Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement
Storage conditions vary
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Limited Use Label Licenses (LULL)
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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser's activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
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Frequently asked questions (FAQs)
The Viral Production Medium, Viral Production Cells 2.0, AAV-MAX Transfection Kit, Viral-Plex Complexation Buffer, and AAV-MAX Lysis Buffer are sold individually and here are the stand-alone catalog numbers:
- Viral Production Medium: Cat. No. A4817901, A4817902, A4817903
- Viral Production Cells 2.0: Cat. No. A49784, A51218
- AAV-MAX Transfection Kit: Cat. No. A50515, A50516
- Viral-Plex Complexation Buffer: A4983901
- AAV-MAX Lysis Buffer: A50520
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Yes, these are different, and each is optimized for its intended use.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.