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Catalog Number | Quantity |
---|---|
K0691 | 50 Preps |
K0692 | 250 Preps |
Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.
The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 μg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.
Highlights
Applications
Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:
We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.
The GeneJET Gel Extraction Kit is designed for gel extraction but can also be used for PCR clean-up by following the protocol from the GeneJET PCR Purification Kit. The components in the GeneJET Gel Extraction Kit and GeneJET PCR Purification Kit are very similar and can be used interchangeably. The exact composition of solutions in the kits is proprietary.
For a 3-in-1 kit for purification of PCR products from enzymatic reaction mixtures and agarose gels, we recommend using the GeneJET Gel Extraction and DNA Cleanup Micro Kit (Cat. Nos. K0831/2).
We recommend performing centrifugation steps at room temperature when the temperature is not specified.
No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
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