Streptomycin Sulfate

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Gibco™

Streptomycin Sulfate

Streptomycin Sulfate is a water-soluble antibiotic originally purified from Streptomyces griseus. Streptomycin Sulfate acts by binding to the 30S subunitRead more

Catalog Number	Quantity
11860038	100 g

Catalog number 11860038

Price (EUR)

204,00

100 g

Add to cart

Quantity:

100 g

Price (EUR)

204,00

100 g

Add to cart

Streptomycin Sulfate is a water-soluble antibiotic originally purified from Streptomyces griseus. Streptomycin Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Streptomycin Sulfate is highly active against gram-negative with some activity against gram-positive bacteria. Gibco™ Streptomycin Sulfate is used alone or in combination with penicillin, an antibiotic highly active against gram-positive bacteria, for the prevention of bacterial contamination of cell cultures. The recommended working concentration ranges from 50 to 100 μg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
For supply chain continuity, we manufacture Gibco™ Streptomycin Sulfate at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration50 to 100 μg/mL

Culture TypeMammalian Cell Culture, Insect Cell Culture

For Use With (Application)Bacterial Selection

Quantity100 g

Shelf Life24 Months

Shipping ConditionRoom Temperature

FormPowder

Product TypeAntibiotic

Unit Size100 g

Contents & Storage

Storage conditions: 2 to 8°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

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Frequently asked questions (FAQs)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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3124658Certificate of AnalysisMay 14, 202511860038

3075977Certificate of AnalysisFeb 23, 202511860038

2701612Certificate of AnalysisJan 31, 202511860038

2715189Certificate of AnalysisJan 29, 202511860038

2701609Certificate of AnalysisJan 29, 202511860038

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Citations & References (5)

Search citations by name, author, journal title or abstract text

Citations & References

Abstract

The cyclin-dependent kinase inhibitor p21WAF1/Cip1 is an antiestrogen-regulated inhibitor of Cdk4 in human breast cancer cells.

Authors: Skildum Andrew J; Mukherjee Shibani; Conrad Susan E;

Journal:J Biol Chem

PubMed ID:11741909

'The MCF-7 cell line is a model of estrogen-dependent, antiestrogen-sensitive human breast cancer. Antiestrogen treatment of MCF-7 cells causes dramatic decreases in both Cdk4 and Cdk2 activities, which leads to a G(1) phase cell cycle arrest. In this report, we investigate the mechanism(s) by which Cdk4 activity is regulated in ... More

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.

Authors: Lund R D; Adamson P; SauvÃ© Y; Keegan D J; Girman S V; Wang S; Winton H; Kanuga N; Kwan A S; BeauchÃ¨ne L; Zerbib A; Hetherington L; Couraud P O; Coffey P; Greenwood J;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11504951

'Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term ... More

Exercise-induced a-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation.

Authors:Yuan Y, Xu P, Jiang Q, Cai X, Wang T, Peng W, Sun J, Zhu C, Zhang C, Yue D, He Z, Yang J, Zeng Y, Du M, Zhang F, Ibrahimi L, Schaul S, Jiang Y, Wang J, Sun J, Wang Q, Liu L, Wang S, Wang L, Zhu X, Gao P, Xi Q, Yin C, Li F, Xu G, Zhang Y, Shu G

Journal:EMBO J

PubMed ID:32104923

'Beneficial effects of resistance exercise on metabolic health and particularly muscle hypertrophy and fat loss are well established, but the underlying chemical and physiological mechanisms are not fully understood. Here, we identified a myometabolite-mediated metabolic pathway that is essential for the beneficial metabolic effects of resistance exercise in mice. We ... More

Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.

Authors:Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM

Journal:EMBO J

PubMed ID:31268602

'The guanylate binding protein (GBP) family of interferon-inducible GTPases promotes antimicrobial immunity and cell death. During bacterial infection, multiple mouse Gbps, human GBP2, and GBP5 support the activation of caspase-1-containing inflammasome complexes or caspase-4 which trigger pyroptosis. Whether GBPs regulate other forms of cell death is not known. The apicomplexan ... More

Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease.

Authors:Wipperman MF, Heaton BE, Nautiyal A, Adefisayo O, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, Krogan N, Glickman MS

Journal:Mol Cell

PubMed ID:30174294

'Infection with Mycobacterium tuberculosis continues to cause substantial human mortality, in part because of the emergence of antimicrobial resistance. Antimicrobial resistance in tuberculosis is solely the result of chromosomal mutations that modify drug activators or targets, yet the mechanisms controlling the mycobacterial DNA-damage response (DDR) remain incompletely defined. Here, we ... More

5 total citations