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Clariom™ S Assay, mouse
Obtain a gene-level view of the mouse transcriptome with the Clariom S Assays for mouse. The Clariom S Assays, mouseRead more
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| Catalog Number | Number of Arrays |
|---|---|
| 902930 | 10 Arrays |
| 902931 | 30 Arrays |
Catalog number 902930
Price (EUR)
2.105,00
Each
Estimated availability date Pending
Number of Arrays:
10 Arrays
Price (EUR)
2.105,00
Each
Obtain a gene-level view of the mouse transcriptome with the Clariom S Assays for mouse. The Clariom S Assays, mouse serve as a next generation transcriptome-wide gene-level expression profiling tool, which allow for the fastest, simplest, and most scalable path to generating actionable results. Based on industry-leading microarray technology, the novel mouse Clariom S Assay design provides extensive coverage of all known well-annotated genes, compatibility with clinical sample types, scalable formats, and flexible data analysis software. Clariom S Assays are the tools of choice to find expression biomarkers with known function as quickly, easily, and cost-effectively as possible.
Find answers, move on
Although the number of known transcribed genes has expanded rapidly in recent years, knowledge of the function of each gene is still evolving. Many genes and transcripts found in databases are poorly annotated or unannotated, which can complicate and prolong data analysis and interpretation. mouse Clariom S Assays focus on well-annotated genes, providing researchers with the ability to perform gene-level expression profiling studies and to quickly assess changes in key genes and pathways. With less time required for data analysis, Clariom S Assays for mouse help researchers reach conclusions more rapidly.
Simple, swift biomarker discovery
• Accurately measure gene-level expression from >20,000 well-annotated genes to get to answers quickly.
• Choose a format that suits your throughput needs, processing from 1 to 192 samples a day.
• Go from data to insight in minutes with intuitive, highly visual, free analysis software designed for the biologist.
When you have precious samples, get it right the first time
• Generate robust expression profiles from as little as 100 pg of total RNA–as few as 10 cells.
• Utilize RNA from various sample types including blood, cells, and fresh/fresh-frozen or FFPE tissues.
• Preserve sample integrity and reduce data variability with an assay that does not require a globin or rRNA removal step.
• Save time and money with fully automated sample preparation options.
Clariom S solutions are available formats for single-sample (cartridge array) processing on the GeneChip™ 3000 instrument system and high-throughput automated processing (plate array) on the GeneTitan™ Microarray System, offering the flexibility to accommodate both small and large cohort studies. The complete solution comes with reagents and fast, simple Transcriptome Analysis Console (TAC) software to analyze and visualize global expression patterns of genes, pathways, and network interactions in minutes.
Get the truest level of gene-level expression
To generate robust gene-level expression, mouse Clariom S Assays detect only the exons present in all known transcript isoforms expressed from a single gene locus-constitutive exons. This differs from other gene-level array technologies and shallow RNA-Seq, which provide either a biased view of gene expression or data that are complicated by variation in expression of transcript variants. By detecting only constitutive exons throughout the length of each known gene, mouse Clariom S Assays generate the most accurate and truest measurement of gene-level expression available today.
Keeping biomarker identification across the transcriptome simple and swift, Clariom S Assays for mouse provide you with the coverage you require, the reproducibility you need, and the insights you want to act on your discoveries.
Find answers, move on
Although the number of known transcribed genes has expanded rapidly in recent years, knowledge of the function of each gene is still evolving. Many genes and transcripts found in databases are poorly annotated or unannotated, which can complicate and prolong data analysis and interpretation. mouse Clariom S Assays focus on well-annotated genes, providing researchers with the ability to perform gene-level expression profiling studies and to quickly assess changes in key genes and pathways. With less time required for data analysis, Clariom S Assays for mouse help researchers reach conclusions more rapidly.
Simple, swift biomarker discovery
• Accurately measure gene-level expression from >20,000 well-annotated genes to get to answers quickly.
• Choose a format that suits your throughput needs, processing from 1 to 192 samples a day.
• Go from data to insight in minutes with intuitive, highly visual, free analysis software designed for the biologist.
When you have precious samples, get it right the first time
• Generate robust expression profiles from as little as 100 pg of total RNA–as few as 10 cells.
• Utilize RNA from various sample types including blood, cells, and fresh/fresh-frozen or FFPE tissues.
• Preserve sample integrity and reduce data variability with an assay that does not require a globin or rRNA removal step.
• Save time and money with fully automated sample preparation options.
Clariom S solutions are available formats for single-sample (cartridge array) processing on the GeneChip™ 3000 instrument system and high-throughput automated processing (plate array) on the GeneTitan™ Microarray System, offering the flexibility to accommodate both small and large cohort studies. The complete solution comes with reagents and fast, simple Transcriptome Analysis Console (TAC) software to analyze and visualize global expression patterns of genes, pathways, and network interactions in minutes.
Get the truest level of gene-level expression
To generate robust gene-level expression, mouse Clariom S Assays detect only the exons present in all known transcript isoforms expressed from a single gene locus-constitutive exons. This differs from other gene-level array technologies and shallow RNA-Seq, which provide either a biased view of gene expression or data that are complicated by variation in expression of transcript variants. By detecting only constitutive exons throughout the length of each known gene, mouse Clariom S Assays generate the most accurate and truest measurement of gene-level expression available today.
Keeping biomarker identification across the transcriptome simple and swift, Clariom S Assays for mouse provide you with the coverage you require, the reproducibility you need, and the insights you want to act on your discoveries.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ArrayTranscriptome Profiling
Number of Arrays10 Arrays
Product LineApplied Biosystems™
Product TypeS Assay
Quantity10 reactions
Shipping ConditionApproved for shipment at Room Temperature or on Wet or Dry Ice
SpeciesMouse
FormatArray Cartridge
Unit SizeEach
Contents & Storage
• Poly-A RNA Control Kit, store at -20°C
• Hybridization Control Kit, store at -20°C
• WT Terminal Labeling Kit, store at -20°C
• WT Amplification Kit, Module 1, store at -20°C
• WT Amplification Kit, Module 2, store at 4°C
• Clariom S 10 Arrays , mouse, store at 4°C
• Hybridization Control Kit, store at -20°C
• WT Terminal Labeling Kit, store at -20°C
• WT Amplification Kit, Module 1, store at -20°C
• WT Amplification Kit, Module 2, store at 4°C
• Clariom S 10 Arrays , mouse, store at 4°C
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Frequently asked questions (FAQs)
Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Tough-Spots® labels are small adhesive stickers used to temporarily seal the backs of cartridge arrays during the overnight hybridization step. They are required to prevent loss of volume due to evaporation through the septa. We recommend using Tough-Spots® labels on Rolls from USA Scientific (Item No. 9185-0000)
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Proper hybridization volume is critical to obtaining an even signal across a given array. Too little volume can lead to black circles in the middle of the array. Too much volume can leak out of the back of the array. The correct hybridization volume leaves enough room for a small air bubble to circulate around the array surface during the overnight hybridization. Here are the recommended hybridization and fill volumes based on the array format:
Array Format; Hybridization Volume; Fill Volume
- 49 Format (Standard); 200 µL; 250 µL
- 64 Format; 200 µL; 250 µL
- 100 Format (Midi); 130 µL; 160 µL
- 169 Format (Mini); 80 µL; 100 µL
- 400 Format (Micro); 80 µL; 100µL
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Clariom D arrays have probes that cover all known regions of transcription including probes in overlapping regions from both strands. To obtain strand-specific information from the Clariom D arrays, the WT Pico and WT Plus reagents (which are strand-specific) must be used. This is important because without strand-specific reagent, it would not be possible to decipher the source strand of DNA, which makes it challenging to untangle true gene- and exon- level expression and alternative splicing events.
Strand-specificity is significantly less important for customers interested in gene-level only information (i.e., those using Clariom S) as compared to customers who want to understand the complexities of the whole transcriptome including identifying antisense transcripts and ncRNA (i.e., those using Clariom D). While strand-specificity is less important for gene-level expression only, probes within regions of overlapping transcription from both strands are avoided in the Clariom S array design (unlike Clariom D). This is important because if Clariom S did not preserve strand-specificity, there could be an overestimation of gene-level expression causing false positive or negative results. With Clariom S having a "stranded" design, it does not necessarily need a strand-specific reagent kit.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
TAC 4.0 includes two algorithms for identifying alternative splicing events: the TAC 2.0 algorithm and the new EventPointer. Algorithmic determination of alternate splicing remains a challenging problem. TAC 4.0 supports two different approaches that have different sets of strengths and weaknesses. After considerable testing, the new TAC 4.0 “'Event Score” leverages both previous TAC 2.0 event estimation score and Event Pointer p-value and sorts the most likely alternative splicing events to the top. Of course, the TAC 2.0 event score and EventPointer p-values remain individually available.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.