Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Features of the Second Strand cDNA Synthesis Kit include: • Efficient synthesis of full-length double-stranded cDNA • Fast—procedure completed in 1 hour • Convenient—pre-mixed components • Complete—includes residual RNA removal reagents
Applications • Full-length double-stranded blunt-end cDNA synthesis for cloning • Double-stranded cDNA library construction
Specifications
Format
50 reactions
Product Type
cDNA Synthesis Kit
For Use With (Application)
Cloning
Final Product Type
Double Stranded cDNA
No. of Reactions
50 Reactions
Reaction Format
Separate Components
Unit Size
50 reaction
Contents & Storage
Contents: • Second Strand Enzyme Mix, 1 mL • 5X Second Strand Reaction Mix, 250 μL • 0.5 M EDTA, pH 8.0, 1 mL • RNase I, 10 U/μL, 500 μL • Water, nuclease-free, 3 x 1.25 mL