Reverse Transcription Products

Reverse Transcription Products

Shop from our array of reverse transcription products designed for efficient RNA-to-cDNA conversion in molecular biology workflows. This category includes cDNA synthesis kits, master mixes, reverse transcriptases, and one-step RT-PCR reagents. These products are essential for various applications, including gene expression analysis, cloning, and pathogen detection. Selection of reverse transcriptases include wild type MMLV-derived enzymes and engineered MMLV RT variants with high thermal stability, reduced RNase H activity and enhanced processivity for improved cDNA yields. cDNA synthesis kits and master mixes provide all components necessary for first-strand cDNA synthesis in a convenient and optimized format. One-step RT-PCR reagents combine reverse transcription and PCR amplification in a single reaction, streamlining workflows for RNA detection and quantification. Learn more about reverse transcription.

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The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA.
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The system includes SuperScript IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and...
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube. It combines the high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR Master Mix to provide...
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified...
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
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Reverse transcription is the process of transcribing RNA molecules into complementary DNA. This cDNA synthesis reaction is mediated by enzymes called reverse transcriptases, or RTs. Moloney murine leukemia virus (M-MLV) reverse transcriptase is one of the RTs used in molecular biology workflows.
Once RNA is quantified, you are ready for reverse transcription. The ideal reverse transcriptase (RT) will reverse-transcribe even the most difficult RNAs, such as those degraded during the purification process or those in limited quantity.
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Product Sheet: TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control
What is the longest cDNA product that Superscript IV RT can synthesize?
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