GeneChip™ 3' IVT Pico Kit

The GeneChip™ 3' IVT Pico Kit (3' IVT Pico Kit) is the most flexible and sensitive target preparation method for accurate and precise analysis of genome-wide expression on 3' IVT arrays from as few as 10 cells.*

Complete flexibility for small quantities of RNA
• Reveal the biology in small subpopulations of cells using as little as 100 pg of total RNA (approximately 10 cells).
• Benefit from total flexibility with a single kit for key sample types:
   - Cultured cells
   - Fresh/fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues
   - Whole blood
• Eliminate the need for globin mRNA or ribosomal RNA reduction to decrease variability and simplify experimental workflows.
• Power clinical research studies by leveraging the unprecedented number of data sets generated using the GeneChip Human Genome U133 array design, including nearly 14,000 publications.
• Preserve precious clinical sample material for future experiments.

Uncover biology hidden in heterogeneous samples
Traditional genome-wide expression analysis requires RNA extracted from samples composed of a relatively large number of cells. However, the gene expression measurements derived from these samples represent averages that can potentially mask key differences between subpopulations of cells within the samples. To better understand the biological significance of these differences in gene expression, researchers are increasingly focused on characterizing and analyzing subpopulations of cells.

Modern technologies, including fluorescence-activated cell sorting (FACS) and laser-capture microdissection (LCM), enable the characterization and subsequent isolation of small populations of cells. However, due to the limited number of cells, these samples typically produce insufficient RNA to obtain robust expression data from microarrays or RNA Sequencing. The 3' IVT Pico Kit, developed specifically to process limited amounts of input RNA, enables researchers to perform robust gene-level analysis on 3' IVT arrays, including GeneChip Human Genome U133 Plus 2.0 Arrays, using just a few cells from a variety of sample types.

A single kit for multiple sample types
Studies often require the analysis of samples derived from multiple types of tissues. Cancer research, for example, commonly involves the comparison of samples derived from fresh frozen tissues and from FFPE tissues. Usually, it is necessary to use different kits to process different sample types, which increases the complexity of the experimental workflow and introduces unwanted variation into the data. The 3' IVT Pico Kit, which can be used to process the majority of sample types used in basic and translational research, streamlines the experimental workflow, and eliminates variability due to different target preparation methods.

Maximize the value of precious samples
Many samples, such as those derived from biopsies, are highly precious because they are unique or otherwise irreplaceable. When working with these precious samples, researchers must focus on preserving the maximum amount of each sample for future experiments. Working with 3' IVT Pico Kit, researchers can use as few as 10 cells to generate robust and reproducible whole-transcriptome data from microarrays, which ensures that the majority of the sample is preserved for future use.

*The recommended amount of input RNA is 500 pg and the minimum amount is 100 pg. Using less than 500 pg of input RNA may result in a slight loss of assay sensitivity. Review GeneChip 3' IVT Pico Reagent Kit Manual Target Preparation for GeneChip 3' Expression Array User Guide (P/N 703308) for more detailed information.