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Invitrogen™
E. coli químicamente competente One Shot™ BL21(DE3)pLysS
Las células BL21(DE3)pLysS son ideales para el uso con sistemas de expresión basados en el promotor T7 (por ejemplo, pRSET,Más información
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| Número de catálogo | Cantidad |
|---|---|
| C606010 | 11 x 50 μl |
| C606003 | 20 x 50 μl |
Número de catálogo C606010
Precio (EUR)
269,00
Each
En Stock
Cantidad:
11 x 50 μl
Precio (EUR)
269,00
Each
Las células BL21(DE3)pLysS son ideales para el uso con sistemas de expresión basados en el promotor T7 (por ejemplo, pRSET, pCR™T7 y pET). Las células E. coli BL21(DE3)pLysS transportan tanto el lisógeno DE3 como el plásmido pLysS. PLysS expresa constitutivamente bajos niveles de lisozima T7, lo que reduce la expresión basal de genes recombinantes al inhibir los niveles basales de ARN polimerasa T7.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Resistencia bacteriana a los antibióticosYes (Chloramphenicol)
Tramado azul/blancoNo
Clonación de ADN metiladoNo
Contiene el episoma F'Carece de episoma F'
Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)
Mejora la calidad de los plásmidosNo
Improves Protein StabilityYes (lon, ompT)
Improves RNA StabilityYes (pLysS)
Preparación de ADN no metiladoYes (dcm)
Línea de productosOne Shot™
Tipo de productoCélula competente
Cantidad11 x 50 μl
Reduce la recombinaciónNo
Condiciones de envíoDry Ice
Resistente al fago T1 (tonA)No
Toxic ProteinsNo
Nivel de eficiencia de transformaciónEficacia media (10^8-10^9 ufc⁄µ g)
FormatoTubo
PromotorT7
EspecieE. coli
Unit SizeEach
Contenido y almacenamiento
• Chemically Competent cells (11 x 50 μL); store at –80°C
• pUC19 Control DNA (1 x 50 μL); store at –80°C
• S.O.C. Medium (6 mL); store at 4°C or room temperature
• pUC19 Control DNA (1 x 50 μL); store at –80°C
• S.O.C. Medium (6 mL); store at 4°C or room temperature
Ready-to-use Bacterial Growth Media
See how these mediums can help with critical aspects of cloning!
Invitrogen One Shot LB Agar* ›
*Only available in North America and selected European countries
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Citations & References (4)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Burkholderia pseudomallei class a beta-lactamase mutations that confer selective resistance against ceftazidime or clavulanic acid inhibition.
Journal:Antimicrob Agents Chemother
PubMed ID:12821450
'Burkholderia pseudomallei, the causative agent of melioidosis, is inherently resistant to a variety of antibiotics including aminoglycosides, macrolides, polymyxins, and beta-lactam antibiotics. Despite resistance to many beta-lactams, ceftazidime and beta-lactamase inhibitor-beta-lactam combinations are commonly used for treatment of melioidosis. Here, we examine the enzyme kinetics of beta-lactamase isolated from mutants
Characterization of the interaction between P143 and LEF-3 from two different baculovirus species: Choristoneura fumiferana nucleopolyhedrovirus LEF-3 can complement Autographa californica nucleopolyhedrovirus LEF-3 in supporting DNA replication.
Journal:J Virol
PubMed ID:14671115
The baculovirus protein P143 is essential for viral DNA replication in vivo, likely as a DNA helicase. We have demonstrated that another viral protein, LEF-3, first described as a single-stranded DNA binding protein, is required for transporting P143 into the nuclei of insect cells. Both of these proteins, along with
Recognition of nonhybridizing base pairs during nucleotide excision repair of DNA.
Journal:Proc Natl Acad Sci U S A
PubMed ID:10339546
Nondistorting C4' backbone adducts serve as molecular tools to analyze the strategy by which a limited number of human nucleotide excision repair (NER) factors recognize an infinite variety of DNA lesions. We have constructed composite DNA substrates containing a noncomplementary site adjacent to a nondistorting C4' adduct to show that
Cloning and characterization of a calcium-binding, histamine-releasing protein from Schistosoma mansoni.
Journal:J Biol Chem
PubMed ID:12050167
A homologue of the mammalian translationally controlled tumor protein (TCTP) was cloned from the human parasite Schistosoma mansoni (SmTCTP). Sequence analysis showed that SmTCTP differed from other reported TCTPs in having only one signature sequence. Subsequently, SmTCTP was cloned in a T7 expression system and expressed as a histidine-tagged fusion
4 total citations