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Invitrogen™
AccuPrime™ Pfx SuperMix
AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Catalog number 12344040
Price (HKD)/ 200 rxns
Price:4,011.00
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Price (HKD)/ 200 rxns
Price:4,011.00
AccuPrime™ Pfx SuperMix
Catalog number12344040
Price (HKD)/ 200 rxns
Price:4,011.00
-
Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.
Features
- >26-times higher fidelity than with Taq DNA polymerase
- Reduced set-up time
- Produces products with blunt ends
Applications
- High-fidelity PCR
- Amplification of fragments up to 15 kb
- Cloning & mutagenesis
Notes
- Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
PolymeraseAccuPrime Pfx DNA Polymerase
Reaction SpeedStandard
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Concentration1.1X
Fidelity (vs. Taq)26X
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Size (Final Product)15 kb or less
Unit Size200 rxns
Contents & Storage
AccuPrime Pfx SuperMix (4 × 1.125 mL)
Store at –20°C in a non-frost-free freezer.
Store at –20°C in a non-frost-free freezer.