Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System. The cells have been adapted to the chemically defined
Viral Production Medium and are maintained in suspension culture in that medium. Viral Production Cells 2.0 are highly transfectable using the
AAV-MAX Transfection Kit for adeno-associated virus (AAV) production.
Features of the Viral Production Cells 2.0 include:
• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5 x 10
10 vg/mL (unpurified)
• Robust scalability and passage stability
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10
7 viable cells/mL in Viral Production Medium and 10% DMSO. Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance.
For Research Use Only. Not for use in diagnostic procedures.