Annexin V Ready Flow Conjugates for Apoptosis Detection
Annexin V Ready Flow Conjugates for Apoptosis Detection
Invitrogen™

Annexin V Ready Flow Conjugates for Apoptosis Detection

Annexin V conjugate stains enable you to easily determine apoptotic cells via flow cytometry.
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Catalog NumberExcitation/EmissionColorConjugate
R37177Promo Image410/455BluePacific Blue
R37174Promo Image495/519GreenAlexa Fluor 488
R37175Promo Image650/665Far-RedAlexa Fluor 647
R37176Promo Image650/660RedAPC (Allophycocyanin)
Catalog number R37177
Price (HKD)
2,399.00
Each
Add to cart
Excitation/Emission:
410/455
Color:
Blue
Conjugate:
Pacific Blue
Recurring order eligible. Learn more »
Price (HKD)
2,399.00
Each
Add to cart
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Enjoy the convenience of ready-to-use Annexin V Ready Flow stand-alone conjugate stains, which offer up to 100-fold fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. There is no need to dilute, weigh, or pipette the conjugate stains; simply add one drop into cells, incubate, and analyze.
Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

The Annexin V Ready Flow conjugates are provided as ready-to-use, direct-to-sample apoptotic cell stains. Simply add one drop from the dropper bottle directly into 1 x 106 cells in 5 mM Ca2+-containing cell culture medium or annexin binding buffer (Cat. Nos. V13246 or BMS500BB), incubate, and analyze. The traditional annexin staining protocol can be used as an alternative method by replacing the addition of 5 μL annexin conjugate with the addition of 1 drop from the Ready Flow dropper bottle.

The Annexin V Alexa Fluor 488 conjugate is excitable at 495 nm with an emission peak at 519 nm.
The Annexin V Alexa Fluor 647 conjugate is excitable at 650 nm with an emission peak at 665 nm.
The Annexin V APC conjugate is excitable at 650 nm with an emission peak at 660 nm.
The Annexin V Pacific Blue conjugate is excitable at 410 nm with an emission peak at 455 nm.
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
DescriptionAnnexin V Pacific Blue Ready Flow Conjugate
Excitation/Emission410/455
Flow Cytometer Laser LinesViolet
For Use With (Equipment)Flow Cytometer
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Quantity120 reactions
Shipping ConditionRoom Temperature
ConjugatePacific Blue
Unit SizeEach
Contents & Storage
Store at room temperature.
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Frequently asked questions (FAQs)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3148245Certificate of AnalysisJul 13, 2025R37177
3123576Certificate of AnalysisApr 14, 2025R37174
3123571Certificate of AnalysisApr 14, 2025R37175
3174481Certificate of AnalysisMar 30, 2025R37176
3001521Certificate of AnalysisOct 08, 2024R37176
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