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Decontaminate clinical specimens prior to inoculation of media for isolating Mycobacterium spp. and fungi using Thermo Scientific™ Remel™ Sodium Citrate 2.94%. Kubica et al. described the N-acetyl-L-cysteine (NAC) method and is the most widely used procedure for digestion and decontamination of clinical specimens1. Reep and Kaplan further investigated the NAC method for the recovery of fungi2. They demonstrated that prior to the inoculation of mycological media, NAC could be used with sodium citrate as a diluent to liquefy specimens.
Most clinical specimens received for isolation of acid-fast bacilli are contaminated with more rapidly growing commensal microbial flora. Respiratory specimens also often contain mucin which may trap the acid-fast bacilli and require liquefaction to release the mycobacterial cells prior to inoculation of media3. Sodium citrate 2.94% helps decontaminate the specimen by eliminating or reducing commensal microbial flora.This reagent contains 2.94% w/v sodium citrate in demineralized water and is supplied in 250mL bottles.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- Kubica, G.P., A. J. Kaufmann, and W.E. Dye. 1963. Amer. Rev. Resp. Dis. 89:284-286.
- Reep, B.R. and W. Kaplan. 1972. Health Lab. Sci. 9:118-124.
- Kent, P.T. and G.P. Kubica. 1985. Public Health Mycobacteriology, A Guide for the Level III Laboratory. U. S. Dept. of H.H.S., CDC, Atlanta, GA.
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