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Invitrogen™
SlowFade™ Diamond Antifade Mountant
SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope深入閱讀
促銷活動優惠代碼:RIRP25
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| 產品號碼 | Volume (Metric) |
|---|---|
| S36963 | 5 x 2 mL |
| S36967 | 2 mL |
| S36972 | 10 mL |
產品號碼 S36963
價格 (HKD)
2,051.00
Each
Volume (Metric):
5 x 2 mL
價格 (HKD)
2,051.00
Each
SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides superior antifade protection across the entire visual and IR spectrums. You can use SlowFade Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background.
Features of SlowFade Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that does not cure; samples can be viewed immediately
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Maintains fluorescence signal—little to no quenching
Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›
For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 μm, try SlowFade Glass Antifade Mountant. It is a RIMS mountant with refractive index of 1.52.
Select the best SlowFade Antifade reagents for your experimental needs ›
SlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can be stored for days to weeks. If long term storage is desired with a refractive index similar to glass or immersion oil, then ProLong Glass Antifade Mountant is recommended. With the no-curing-required properties of SlowFade Diamond mountant, samples can be evaluated and imaged right after labeling.
Get photobleaching protection across the entire visible and IR spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, SlowFade Diamond mountant helps provide the best protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. SlowFade Diamond mountant does not quench, and its ability to provide brighter signal, against no background, helps ensure that you obtain the best-quality, high resolution images for publishing.
Stable signals during imaging and for immediate viewing
SlowFade Diamond mountant is the ideal option for analyzing samples right after labeling. Its antifade properties for both fluorescent dyes and fluorescent proteins preserves signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. A slide sealant can be used to preserve the slides for days or weeks. If longer archiving is desired, then the SlowFade Diamond mountant can be washed away with PBS, and the samples remounted with ProLong Diamond Antifade Mountant.
Achieve excellent refractive index for crisp images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. SlowFade™ Diamond mountant has a refractive index of 1.42 and is recommended for cultured cells or thin slices of tissue (less then 10 μm in thickness).
Features of SlowFade Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that does not cure; samples can be viewed immediately
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Maintains fluorescence signal—little to no quenching
Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›
For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 μm, try SlowFade Glass Antifade Mountant. It is a RIMS mountant with refractive index of 1.52.
Select the best SlowFade Antifade reagents for your experimental needs ›
SlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can be stored for days to weeks. If long term storage is desired with a refractive index similar to glass or immersion oil, then ProLong Glass Antifade Mountant is recommended. With the no-curing-required properties of SlowFade Diamond mountant, samples can be evaluated and imaged right after labeling.
Get photobleaching protection across the entire visible and IR spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, SlowFade Diamond mountant helps provide the best protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. SlowFade Diamond mountant does not quench, and its ability to provide brighter signal, against no background, helps ensure that you obtain the best-quality, high resolution images for publishing.
Stable signals during imaging and for immediate viewing
SlowFade Diamond mountant is the ideal option for analyzing samples right after labeling. Its antifade properties for both fluorescent dyes and fluorescent proteins preserves signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. A slide sealant can be used to preserve the slides for days or weeks. If longer archiving is desired, then the SlowFade Diamond mountant can be washed away with PBS, and the samples remounted with ProLong Diamond Antifade Mountant.
Achieve excellent refractive index for crisp images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. SlowFade™ Diamond mountant has a refractive index of 1.42 and is recommended for cultured cells or thin slices of tissue (less then 10 μm in thickness).
For Research Use Only. Not for use in diagnostic procedures.
規格
Quantity5 x 2 mL
Product LineSlowFade™
Product TypeAntifade Mountant
Reagent TypeMounting Solution, Antifade Solution
Volume (Metric)5 x 2 mL
Unit SizeEach
內容物與存放
Contains 5 dropper bottles contaning 2 mL reagent each.
• Store at 2-8°C or frozen at -5 to -30°C.
• Protect from light.
• Stable for at least 6 months after receiving.
• Store at 2-8°C or frozen at -5 to -30°C.
• Protect from light.
• Stable for at least 6 months after receiving.
Running Fluorescence-based assays?
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圖表
Photobleaching resistance for fluorescent dyes and proteins with SlowFade® antifade reagents
Enhanced resistance to photobleaching afforded by SlowFade® antifade reagents
HeLa cells mounted with SlowFade® Diamond mountant
HeLa cells mounted with SlowFade® Diamond mountant
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文件與下載
證書
依批號或部分批號搜尋
批號Certificate TypeDateCatalog Number(s)
3206096Certificate of AnalysisJun 29, 2025S36972
3136932Certificate of AnalysisMay 18, 2025S36967
3079806Certificate of AnalysisDec 22, 2024S36972
2985052Certificate of AnalysisDec 07, 2024S36963
2985045Certificate of AnalysisOct 31, 2024S36972
顯示 5 個結果, 在上方搜尋特定憑證
安全資料表
SDS常見問答集 (常見問題)
Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.
When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.
When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
As dyes are illuminated for imaging, they will fade, or “photobleach”, leading to unwanted dimming and lower detection efficiency over time. An antifade mounting medium can greatly reduce photobleaching. If you wish to label live cells, use of ProLong Live Antifade Reagent is helpful. If you wish to mount fixed cells after labeling, and then image immediately and then discard, SlowFade Diamond Antifade Mountant stays liquid but has good refractive index. If you wish to mount your sample and then archive the slides, ProLong Diamond Antifade Mountant will harden to a better refractive index and allow for archiving of the sample for up to several weeks, or even months. Unlike other antifade mounting media, these work well with fluorescent proteins for immediate viewing (archiving fluorescent proteins is not possible), and they are packaged with or without DAPI. More information on these can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades/prolong-gold-antifade.html).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
引用資料與參考文獻 (21)
Search citations by name, author, journal title or abstract text
引用資料與參考文獻
Abstract
Involvement of stress-related genes polB and PA14_46880 in biofilm formation of Pseudomonas aeruginosa.
Journal:
PubMed ID:25156741
Chronic infections of Pseudomonas aeruginosa are generally established through production of biofilm. During biofilm formation, production of an extracellular matrix and establishment of a distinct bacterial phenotype make these infections difficult to eradicate. However, biofilm studies have been hampered by the fact that most assays utilize nonliving surfaces as biofilm
Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors.
Journal:Cell Rep
PubMed ID:31875561
'In vitro gametogenesis is the process of making germline cells from human pluripotent stem cells. The foundation of this model is the quality of the first progenitors called primordial germ cells (PGCs), which in vivo are specified during the peri-implantation window of human development. Here, we show that human PGC (hPGC) specification
ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples.
Journal:Sci Rep
PubMed ID:28176832
'Investigations into the epigenetic status of individual cells within tissues can produce both epigenetic data for different cell types and positional information of the cells. Thus, these investigations are important for understanding the intra- and inter-cellular control systems of developmental and environmental responses in plants. However, a simple method to
Revealing hidden complexities of genomic rearrangements generated with Cas9.
Journal:Sci Rep
PubMed ID:28993641
'Modelling human diseases caused by large genomic rearrangements has become more accessible since the utilization of CRISPR/Cas9 in mammalian systems. In a previous study, we showed that genomic rearrangements of up to one million base pairs can be generated by direct injection of CRISPR/Cas9 reagents into mouse zygotes. Although these
SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.
Journal:Mol Microbiol
PubMed ID:31290194
'Inhibition of cell division is critical for viability under DNA-damaging conditions. DNA damage induces the SOS response that in bacteria inhibits cell division while repairs are being made. In coccoids, such as the human pathogen, Staphylococcus aureus, this process remains poorly studied. Here, we identify SosA as the staphylococcal SOS-induced
21 total citations