Informational:Notice: Change in the component name! We're enhancing our manufacturing process for DNA Control Template, now labelled as pREF-BDT Control DNA in kit, for consistent high-quality delivery. No changes to preparation or storage conditions will occur ensuring seamless integration into your current workflows. Please see the product user guide for more information.
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT-rich regions • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Description
Cycle Sequencing Kit, 5000 rxns, Includes 2 x 20mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, 2 tubes M13 (-21) Primer, 2 tubes pGEM Control DNA, 2 x 28mL tubes of 5X Sequencing Buffer (may be stored at 4°C)
Format
12-well Strips, 8-well Strip, 96- or 384-well Plate
Ideal for De Novo Sequencing, Resequencing and Finishing with PCR Product, Plasmid, Fosmid and BAC templates
Includes
2 x 20mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, 2 tubes M13 (-21) Primer, 2 tubes pGEM Control DNA, 2 x 28mL tubes of 5X Sequencing Buffer (may be stored at 4°C)
No. of Reactions
5000
Product Line
BigDye™ Terminator, BigDye™
Product Type
Sequencing Buffer
Technique
Fluorescent Dye Terminator Sequencing
Template Compatibility
BAC DNA, Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA
Contents & Storage
• 2 × 20-ml tubes of BigDye™ Terminator v3.1 Ready Reaction Mix • 2 tubes M13 (-21) Primer • 2 tubes pGEM Control DNA • 2 × 28-ml tubes of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.