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BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishingRead more
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| Catalog Number | Quantity |
|---|---|
| 4337455 | 100 Reactions |
| 4337454 | 24 Reactions |
| 4337456 | 1000 Reactions |
| 4337457 | 5000 Reactions |
| 4337458 | 25,000 Reactions |
Catalog number 4337455
Price (USD)Request A Quote
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Quantity:
100 Reactions
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
- Improve the quality of your results for a wide range of sequencing applications
- Optimized for long read lengths
- Better dye mobility characteristics
- Improved performance reading through GT-rich regions
- Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
- Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
For Use With (Equipment)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Product LineBigDye™ Terminator
Product TypeSequencing Buffer
Quantity100 Reactions
TechniqueFluorescent Dye Terminator Sequencing
Template CompatibilityBAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons
Format12-well Strips, 8-well Strip, 96- or 384-well Plate
Unit SizeEach
Contents & Storage
• 1 x 800 μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix
• 1 tube M13 (-21) Primer
• 1 tube pREF-BDT™ Control DNA
• 2 x 1 mL tubes of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
• 1 tube M13 (-21) Primer
• 1 tube pREF-BDT™ Control DNA
• 2 x 1 mL tubes of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
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Limited Use Label License No. 437: Sequencing
Notice to Purchaser: This product is sold pursuant to a limited sublicense from Amersham International. The purchase of this product includes a limited non-exclusive sublicense (without the right to resell, repackage or further sublicense) under such patent rights to use this reagent for DNA sequencing or fragment length analysis solely with a Life Technologies commercial automated sequencing machine or other authorized D NA sequencing machines that have been authorized for such use by Life Technologies, or for manual DNA sequencing. No license is hereby granted for use of this product or the reagents therein, in any other automated sequencing machine. Such sublicense is granted solely for research and other uses that are not unlawful. No other license is granted expressly, impliedly, or by estoppel. For information concerning the availability of additional license to practice the patented methodologies, contact: Amersham Life Science, Inc. , Vice President, Regulatory Affairs, P. O. Box 22400, Cleveland, Ohio 441 22 {Tel: (216) 765-500}.
Frequently asked questions (FAQs)
When contacting Genetic Analysis Support (FAS, FSE, Technical Support), please have (if possible):
Instrument model and serial numbers
Version of the software are you using
Application and/or kit information
Kit lot number
Number of runs on the capillary or array
Polymer type and lot number
Buffer lot number
Hi-Di Formamide lot number or loading solution information
Sample data that can be sent to the support person
Details on:
-Reaction setup (i.e., how much DNA was used, primer, etc.)
-How the reactions were cleaned up (alcohol precipitation, columns, etc)
-What template DNA was used (i.e. plasmid, PCR product)
-How the template DNA was prepared?
All of this information will help the support person quickly and accurately assess the problem and provide you with recommendations for resolution.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.
In order to get through homopolymeric regions, you can either use anchored sequencing primers or try using the dRhodamine Terminator Cycle Sequencing Kit since it uses ddTTP instead of ddUTP and has been shown to be less prone to producing stutters, specifically with poly-T regions.
For more information on sequencing through homopolymer stretches, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.
When trying to sequence through GC-rich regions, the following tips may improve your success:
• Set the reaction up at 1X or 0.5X strength. Heavily diluting the BigDye Terminator Ready Reaction mix can reduce the success of the sequecing reaction through long GC stretches.
• Perform a hot start at 98–99°C for 5 minutes.
• Use 5% (w/v) DMSO or freshly made betaine in the reaction.
• Use the dGTP BigDye Terminator Cycle Sequencing Kit. G peaks will appear compressed due to the presence of ddGTP, but sequencing through long GC stretches using the dGTP kit is typically more successful than with the standard BigDye Terminator Cycle Sequencing Kits (containing ddITP).
For more information on sequencing GC-rich DNA, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.
Some of the causes of another sequence appearing under the primary sequence are:
• Contamination: There is more than one species of DNA present (e.g., multiple PCR products).
• Primers: Primer is annealing in more than one location on the template, primer dimer, primer degradation or not manufactured properly resulting in N+1 or N-1, carry over from PCR reaction, or primers pairs in a multiplex reaction that are not appropriate for multiplexing (i.e., primers anneal inappropriately).
• Spectral/Matrix: If the raw data signal intensity of the sample is too high or saturated it can exceed the amount of color bleedthrough (or spectral overlap) that the matrix (310) or spectral (3130, 3730, 3500) are removing, resulting in secondary peaks appearing in a very specific pattern (e.g., a red peak always appearing under a green peak). A change in the camera, laser, or optical alignment requires that a new matrix be made or a new spectral calibration be performed.
For more information on more than one sequence or set of peaks in a sequencing run, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.
The cause of this is usually an overabundance of template DNA relative to the amount of BigDye Terminator Ready Reaction mix being used in the reaction. This can drive the reaction to incorporate the labeled ddNTPs closer to the 5’ end of the sequence. To balance the signal, either increase the amount of BigDye Terminator Ready Reaction mix being used in the reaction or decrease the concentration of the template DNA.
For more information on other causes of short reads and how to address each issue, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: Second Edition (Cat. No. 4305080, Rev. C). The guide can be found by searching the Thermo Fisher Scientific website with the catalog number 4305080.
Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.