PichiaPink™ Vector Kit
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Invitrogen™

PichiaPink™ Vector Kit

The PichiaPink™ Vector Kit contains the pPINK-HC and pPINK-LC vectors for use with the PichiaPink™ Yeast Expression System. The pPINKRead more
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Catalog NumberQuantity
A111521 kit
Catalog number A11152
Price (KRW)
2,514,000
Each
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Quantity:
1 kit
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Price (KRW)
2,514,000
Each
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The PichiaPink™ Vector Kit contains the pPINK-HC and pPINK-LC vectors for use with the PichiaPink™ Yeast Expression System. The pPINK vectors are built around the ADE2 gene for complementing the ade2 deletion in the PichiaPink™ strains. You can order the PichiaPink™ Vector Kit to refill your PichiaPink™ Yeast Expression System Kits.

The PichiaPink™ Vector Kit comes with:
•     pPINK-LC vector (low-copy number) (20 μg)
•     pPINK-HC vector (high-copy number) (20 μg)(1)
•     5’AOX1 sequencing primer (20 μg)
•     3’CYC3 sequencing primer (20 μg)
•     One Shot™ TOP10 Electrocomp™ E. coli (Cat. No. C404052)

Two Vector Options
The PichiaPink™ Vector Kit comes with two vectors: pPINK-LC (low-copy number) and pPINK-HC (high-copy number). You can choose the vector that works better at producing your protein. Both vectors use the methanol-induced AOX1 promoter to express your protein.

Both pPINK-LC and pPINK-HC vectors are compatible with the PichiaPink™ Secretion Signal Set. Using a 3-way ligation, you can add one of 8 secretion sequences to your gene. The pPINK vectors express proteins intracellularly by default.

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (2,3). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
Download the pPINK-LC plasmid map (PDF).
Download the pPINK-HC plasmid map (PDF).
Learn more about the PichiaPink™ Yeast Expression System.
Learn more about our other protein expression systems.

References

1. Du, et al. (2012) Bioengineered Bugs 3:1, 32-37.
2. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
3. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeVector Kit
Quantity1 kit
VectorpPINK-HC, pPINK-LC
Unit SizeEach
Contents & Storage
The PichiaPink™ Vector kit contains high- and a low copy number plasmids: pPink-HC and pPink-LC (Quantity: 20 μg each). Sequencing primers (5' AOX1 and 3'CYC1) are included. Store at -20°C
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Vector Information

Vector Name
Vector Map
Polylinker
Sequence
Restriction
pPIC6
pMT/V5-His

Product Information

Limited Use Label Licenses (LULL)

Notice to Purchaser: This product is produced subject to an exclusive license to Life Technologies Corporation for U.S. Patents and foreign equivalents owned or controlled by MERCK & Co., Inc. (the "MERCK Patents"), and related technology. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product, including its progeny and derivatives, and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity) as set forth in the accompanying product instruction manual. By purchase and use of this product, the buyer agrees to grant to MERCK & Co., Inc. and its AFFILIATES a non-exclusive, non-assignable, non-sublicensable, fully paid up, royalty-free license to all Patented Improvements made by or on behalf of the buyer through the use of this product. "Patented Improvements" means any modification of this product or its use (a) that is the subject of an issued claim in a patent, wherein the issued claim is directed to an invention that was conceived by the buyer or a person under the direction or supervision of the buyer, and (b) which was developed through the use of this product. For clarity, any technology that was developed without the employment of this product shall not constitute a Patented Improvement, and any protein, including any recombinant protein, expressed through use of this product shall not constitute a Patented Improvement. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research under the terms of this Limited Use Label License and not for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For products that are subject to multiple limited use label licenses, the most restrictive limited use label license terms apply. The research use limitation stated above shall not preclude the buyer from the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed, provided that (i) neither this product nor any of its components was used in the manufacture of such product, and (ii) the product or its components are not included in the therapeutic, prophylactic or diagnostic product. If the buyer is not willing to accept the limitations of this limited use statement, Life Technologies is willing to accept return of the product for a full refund. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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