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Catalog Number | Unit Size | Price (KRW) | Availability | Quantity | |
---|---|---|---|---|---|
EP0451 | 10,000 units | Price: 171,000 Online offer: 158,000 (ends 01-Oct-2024) Your price: | - | ||
EP0452 | 5 x 10,000 units | Price: 658,000 Online offer: 593,000 (ends 01-Oct-2024) Your price: | - |
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).
Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension
• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer
Store at –20°C.