The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ ready-to-use panels and custom primer pools, for sequencing on the Ion Personal Genome Machine™ (PGM™) System. This new 'LV' version of the kit is configured for large reagent volumes, making it ideal for automated DNA library preparation protocols; it can also be used with manual protocols. This new version replaces the previous 'non-LV' version (Cat # 4478378).

Key Features:

New Simple Protocol
• 96-well plate-based workflow allows easy sample management
• Only 1 required cleanup step reduces hands-on-time
• No library amplification with qPCR quantification

New Ion AmpliSeq™ HiFi Master Mix
• Enables cleaner amplification for increased coverage uniformity
• Up to 3,072-plex PCR allows larger panel designs

New Digestion Reagent
• Cleaner digest allows for more efficient downstream sequencing

Scalable Multiplex PCR Reactions
The Ion AmpliSeq™ 2.0 technology enables scalable multiplex PCR reactions from 12- to 3,072-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq™ panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™ Custom Primer Pools are designed via the Ion AmpliSeq™ Designer web-hosted software, available at www.ampliseq.com.

Barcoded Library Preparation
The Ion AmpliSeq™ Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq™ primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1–96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq™ Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation is completed in as little as 3.5 hours using 10 ng of DNA per PCR, and DNA from a variety of sources—including formalin-fixed, paraffin-embedded (FFPE) tissue can be used as the starting material.

The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ Particles using the automated Ion OneTouch™ System. The intuitive Torrent Suite Software and optional Ion Reporter enable you to go from extracted DNA to variant calls in as little as 10 hours.