Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
카탈로그 번호 | 수량 |
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88315 | 16 x 12.9 μg |
ActivX™ Desthiobiotin-GTP Probes contain a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. ActivX active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to allow profiling of both inactive and active enzymes. The ActivX probes selectively enrich only those enzymes that are functionally active and biologically relevant at the time of labeling. With selective enrichment, one can identify and profile target enzyme classes across samples or can assess the specificity and affinity of enzyme inhibitors.
After removal of GTP or GDP nucleotides from enzymes, the desthiobiotin-GTP probe can be used to covalently modify conserved lysine residues in the GTPase nucleotide-binding site. Desthiobiotin-GTP can selectively enrich, identify and profile target enzyme classes in samples. Pre-incubation of samples with small-molecule inhibitors that compete with active-site probes can be used to determine inhibitor binding affinity and target specificity. The ActivX Desthiobiotin-GTP Probe covalently labels the active site of GTPases and GTPase subunits of G-protein coupled receptors.
Applications of ActivX Desthiobiotin-GTP Probe:
• Broad enrichment of GTP-binding proteins from tissues, cells and sub-cellular proteomes
• Enrichment of enzymes based on function
• Profiling of dozens to hundreds of inhibitor targets
Assessment of active-site labeling with desthiobioin probes can be accomplished by either Western blot or mass spectrometry (MS). For the Western blot workflow, desthiobiotin-labeled proteins are enriched for SDS-PAGE analysis and subsequent detection with specific antibodies. For the MS workflow, desthiobiotin-labeled proteins are reduced, alkylated and enzymatically digested to peptides. Only the desthiobiotin-labeled, active-site peptides are enriched for analysis by LC-MS/MS. Both workflows can be used for determining inhibitor target binding, but only the MS workflow can identify global inhibitor targets and off-targets.
These products are subject to a limited use label license.
Buyer may not sell, transfer or otherwise provide access to (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.
The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator is provided with a copy of this Limited Use Label License and agrees to be bound by its terms.
Commercial Purposes means any activity by a party for monetary or other consideration and includes, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes—including the search for new drugs, or new discovery methods and to research, develop, or derive products ; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research.
These products and product applications are covered by applicable U.S. and foreign patents and patents pending.
Recipient agrees not to reformulate, alter or make any changes to the Probe without prior written permission from ActivX Biosciences, Inc.
If the buyer is not willing to accept the limitations of this limited use statement, Pierce Biotechnology, Inc. is willing to accept return of the unused product with a full refund. By opening and using this product, the buyer agrees to be bound by the conditions of this Limited Use Label License.
Notwithstanding the restrictions set forth herein, buyer may use the product(s) for any Commercial Purpose with a valid license from ActivX Biosciences, Inc. For information on acquiring a license to this product for purposes other than those set forth herein, contact proteomics.licensing@thermofisher.com, Pierce Biotechnology, Inc., 3747 North Meridian Road, Rockford, IL 61101 or ActivX Biosciences, Inc. (info@activx.com).
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