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설명 | iodoTMTsixplex™ Isobaric Label Reagent Set, 5 x 0.2 mg |
용도(장비) | Mass Spectrometer |
제품라인 | iodoTMTsixplex™ |
라벨 또는 염료 | Tandem Mass Tag™ (TMT) Labels |
시작 물질 | Cell Lysate |
최종 제품 유형 | Protein |
검출 방법 | Mass Spectrometry |
워크플로우 단계 | Protein Labeling |
수량 | 5 x 0.2 mg (per tag) |
Unit Size | 5 x 0.2 mg (per tag) |
설명 | iodoTMTzero™ Label Reagent, 5 x 0.2 mg |
용도(장비) | Mass Spectrometer |
제품라인 | iodoTMTzero™ |
라벨 또는 염료 | Tandem Mass Tag™ (TMT) Labels |
시작 물질 | Cell Lysate |
최종 제품 유형 | Protein |
검출 방법 | Mass Spectrometry |
워크플로우 단계 | Protein Labeling |
수량 | 5 x 0.2 mg |
Unit Size | 5 x 0.2 mg |
설명 | iodoTMTsixplex™ Isobaric Label Reagent Set, 1 x 0.2 mg |
용도(장비) | Mass Spectrometer |
제품라인 | iodoTMTsixplex™ |
라벨 또는 염료 | Tandem Mass Tag™ (TMT) Labels |
시작 물질 | Cell Lysate |
최종 제품 유형 | Protein |
검출 방법 | Mass Spectrometry |
워크플로우 단계 | Protein Labeling |
수량 | 1 x 0.2 mg (per tag) |
Unit Size | 1 x 0.2 mg (per tag) |
카탈로그 번호 | 사양 | 제품 사이즈 | 설명 | 제품 정가(KRW) | 재고 정보 | 수량 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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90102 | 5 x 0.2 mg (per tag) | iodoTMTsixplex™ Isobaric Label Reagent Set, 5 x 0.2 mg | 제품 정가 3,825,000 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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90100 | 5 x 0.2 mg | iodoTMTzero™ Label Reagent, 5 x 0.2 mg | 제품 정가 325,000 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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90101 | 1 x 0.2 mg (per tag) | iodoTMTsixplex™ Isobaric Label Reagent Set, 1 x 0.2 mg | 제품 정가 1,075,000 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Iodoacetyl tandem mass tag reagents enable selective labeling of cysteine-containing peptides for multiplexed sample quantitation using LC-MS/MS analysis. Similar to iodoacetamide, iodoTMT reagents react specifically with reduced cysteines (Cys) in peptides and proteins and enable quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation and the oxidation of disulfide bonds. IodoTMT reagents irreversibly label free sulfhydryl groups on cysteine residues, in contrast to previous reversible cysteine-reactive TMT (cysTMT) reagents.
To selectively analyze the cysteine-labeled peptides, the sample may be enriched using Immobilized Anti-TMT Antibody Resin (Cat. No. 90076) and TMT Elution Buffer (Cat. No. 90104). This approach of selective thiol-labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT method) but allows for affinity enrichment with a non-biological tag and higher multiplex quantitation.
Features of iodoTMT reagents
• Specific—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
• Irreversible—labeled proteins and peptides are not susceptible to reducing agents
• Flexible—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
• Versatile—can be used to study cysteine modifications (e.g., di-sulfide bridges, S-nitrosylation)
General References:
Thompson, A., et al. (2003). Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS. Anal. Chem. 75:1895-204.
Forrester MT, et al. (2009). Detection of protein S-nitroylsation with the biotin technique. Free Radic Biol Med. 46(2):119-126.
Gygi, S.P., et al. (1999). Quantitative analysis of complex protein mixtures using isotopecoded affinity tags. Nat. Biotech. 17:994-9.
Murray CI, et al. (2012) Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay. Mol Cell Proteomics. 11(2): M111.013441.
Yao, C., et al. (2013). Proteomic study of reversible cysteine oxidation in catalase overexpressing mice on a western diet. FASEB J, Apr 2013; 27:810.3.