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Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents
Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents
Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
사양
형식
200 reactions
제품 유형
cDNA Synthesis Kit
용도(애플리케이션)
Cloning
최종 제품 유형
Double Stranded cDNA
반응 수
200 Reactions
반응 형식
Separate Components
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Contents:
• Second Strand Enzyme Mix, 4 x 1 mL
• 5X Second Strand Reaction Mix, 4 x 250 μL
• 0.5 M EDTA, pH 8.0, 4 x 1 mL
• RNase I, 10 U/μL, 4 x 500 μL
• Water, nuclease-free, 12 x 1.25 mL
Storage: -15 to -25°C
• Second Strand Enzyme Mix, 4 x 1 mL
• 5X Second Strand Reaction Mix, 4 x 250 μL
• 0.5 M EDTA, pH 8.0, 4 x 1 mL
• RNase I, 10 U/μL, 4 x 500 μL
• Water, nuclease-free, 12 x 1.25 mL
Storage: -15 to -25°C
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