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CellLight™ Mitochondria-GFP, BacMam 2.0
본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기
CellLight™ Mitochondria-GFP, BacMam 2.0
Invitrogen™

CellLight™ Mitochondria-GFP, BacMam 2.0

CellLight Mitochondria-GFP, BacMam 2.0, provides an easy way to label mitochondria with green fluorescent protein (GFP) in live cells. Simply자세히 알아보기
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카탈로그 번호수량
C10600Promo Image1 mL
카탈로그 번호 C10600
제품 가격(KRW)
769,000
Online offer
Ends: 30-Sep-2025
904,000
할인액 135,000 (15%)
Each
재고 있음
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수량:
1 mL
Recurring order eligible. Learn more »
제품 가격(KRW)
769,000
Online offer
Ends: 30-Sep-2025
904,000
할인액 135,000 (15%)
Each
카트에 추가하기
Ask our AI about this Product
CellLight Mitochondria-GFP, BacMam 2.0, provides an easy way to label mitochondria with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the leader sequence of E1 alpha pyruvate dehydrogenase. You can observe mitochondria-GFP behavior in live cells independently of mitochondrial membrane potential and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight Technology is:
Fast and convenient: simply add CellLight reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight Mitochondria-GFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and emGFP, providing accurate and specific targeting to cellular mitochondria-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
사양
색상Green
검출 방법Fluorescence
염료 유형GFP (EmGFP)
방출Visible
여기 파장 범위488⁄510
용도(장비)Confocal Microscope, Fluorescence Microscope
형태Liquid
제품라인CellLight™
수량1 mL
배송 조건Wet Ice
기술Fluorescence Intensity
라벨 유형Fluorescent Dye
제품 유형Mitochondria Probe
SubCellular LocalizationMitochondria
Unit SizeEach
구성 및 보관
Store at 2°C to 6°C, protected from light. Do Not Freeze.
Have questions about this product? Ask our AI assisted search.
What safety precautions need to be taken to work with the BacMam reagent?
Are the CellLights products toxic to cells?
Do CellLight BacMam 2.0 reagents work on all cell types?
I keep getting low transduction efficiency when using CellLight labeling reagents on my neurons. What can I do to improve the efficiency?
How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?
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그림

형광 스펙트럼

Fluorescence spectra

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문서 및 다운로드

Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
2659278Certificate of Analysis2023년 9월 06일C10600
2559158Certificate of Analysis2022년 11월 29일C10600
2488983Certificate of Analysis2022년 6월 29일C10600
2392728Certificate of Analysis2021년 9월 13일C10600
2277658Certificate of Analysis2021년 2월 11일C10600
시험 성적서 관련 5 개의 결과가 검색되었습니다.

Safety Data Sheets | 물질 안전 보건 자료

Molecular Probes® 핸드북

자주 묻는 질문(FAQ)

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Cells transduced with the CellLights reagents can be stored frozen for several months after transduction, without loss of expression.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If the viral particles are used at the level we recommend, they are very well tolerated by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The BacMam 2.0 CellLights typically express for 5 days after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The first generation BacMam reagents were shown to efficiently transduce over 90 cell types, including stable cell lines and primary cells. With BacMam 2.0, it is now possible to also efficiently transduce primary neurons and stem cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (30)

인용 및 참조 문헌
Abstract
Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function.
Authors:Chevrollier A, Cassereau J, Ferré M, Alban J, Desquiret-Dumas V, Gueguen N, Amati-Bonneau P, Procaccio V, Bonneau D, Reynier P,
Journal:Int J Biochem Cell Biol
PubMed ID:22433900
'Mitochondria form dynamic tubular networks through processes of fission and fusion. Defect in mitochondrial dynamics lead to various pathologies, including several common and some rare neurodegenerative disorders. OPA1 and MFN2 are two key players in mitochondrial fusion associated with Autosomal Dominant Optic Atrophy and Charcot Marie Tooth neuropathy type 2A ... More
Ketamine induces toxicity in human neurons differentiated from embryonic stem cells via mitochondrial apoptosis pathway.
Authors:Bosnjak ZJ, Yan Y, Canfield S, Muravyeva MY, Kikuchi C, Wells CW, Corbett JA, Bai X,
Journal:Curr Drug Saf
PubMed ID:22873495
'Ketamine is widely used for anesthesia in pediatric patients. Growing evidence indicates that ketamine causes neurotoxicity in a variety of developing animal models. Our understanding of anesthesia neurotoxicity in humans is currently limited by difficulties in obtaining neurons and performing developmental toxicity studies in fetal and pediatric populations. It may ... More
Energy metabolism in human pluripotent stem cells and their differentiated counterparts.
Authors:Varum S, Rodrigues AS, Moura MB, Momcilovic O, Easley CA, Ramalho-Santos J, Van Houten B, Schatten G,
Journal:PLoS One
PubMed ID:21698063
'BACKGROUND: Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless their use may prove challenging as incomplete differentiation of these cells might lead ... More
Nitrite activates protein kinase A in normoxia to mediate mitochondrial fusion and tolerance to ischaemia/reperfusion.
Authors:Pride CK, Mo L, Quesnelle K, Dagda RK, Murillo D, Geary L, Corey C, Portella R, Zharikov S, St Croix C, Maniar S, Chu CT, Khoo NK, Shiva S,
Journal:
PubMed ID:24081164
'Nitrite (NO2(-)), a dietary constituent and nitric oxide (NO) oxidation product, mediates cardioprotection after ischaemia/reperfusion (I/R) in a number of animal models when administered during ischaemia or as a pre-conditioning agent hours to days prior to the ischaemic episode. When present during ischaemia, the reduction of nitrite to bioactive NO ... More
Inhibition of AMP-activated protein kinase a (AMPKa) by doxorubicin accentuates genotoxic stress and cell death in mouse embryonic fibroblasts and cardiomyocytes: role of p53 and SIRT1.
Authors:Wang S, Song P, Zou MH,
Journal:J Biol Chem
PubMed ID:22267730
'Doxorubicin, an anthracycline antibiotic, is widely used in cancer treatment. Doxorubicin produces genotoxic stress and p53 activation in both carcinoma and non-carcinoma cells. Although its side effects in non-carcinoma cells, especially in heart tissue, are well known, the molecular targets of doxorubicin are poorly characterized. Here, we report that doxorubicin ... More
30 total citations

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