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Invitrogen™
Anza™ 12 XbaI
5' T ↓ C T A G A 3'3' A G A T C ↑ T 5'Invitrogen™ Anza™ 12 XbaI자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| IVGN0128 | 12,000 units |
| IVGN0126 | 2000 units |
카탈로그 번호 IVGN0128
제품 가격(KRW)
569,000
Each
-
수량:
12,000 units
제품 가격(KRW)
569,000
Each
5' T ↓ C T A G A 3'
3' A G A T C ↑ T 5'
Invitrogen™ Anza™ 12 XbaI is a restriction enzyme that cuts DNA at this recognition site: T^CTAGA, completely digesting the DNA in 15 minutes at 37°C.
For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.
• Convenient—all Anza enzymes are 100% functional in the single Anza buffer
• Fast—complete digestion in 15 minutes
• Flexible—overnight digestion without star activity
• Simple—a single digestion protocol for all DNA types
• Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience
Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.
Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
3' A G A T C ↑ T 5'
Invitrogen™ Anza™ 12 XbaI is a restriction enzyme that cuts DNA at this recognition site: T^CTAGA, completely digesting the DNA in 15 minutes at 37°C.
For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.
• Convenient—all Anza enzymes are 100% functional in the single Anza buffer
• Fast—complete digestion in 15 minutes
• Flexible—overnight digestion without star activity
• Simple—a single digestion protocol for all DNA types
• Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience
Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.
Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.
사양
함께 사용가능한 버퍼Anza™ 10X Red Buffer, Anza™ 10X Buffer
제품 유형Restriction Enzyme Cloning System
수량12,000 units
배송 조건Approved for shipment on Wet or Dry Ice
효소Xba I
메틸화 민감도Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
최적 반응 온도37°C
제품라인Anza™
연구 카테고리Traditional Cloning
열 불활성화에 대한 민감성Yes
Type IIS RENo
Unit SizeEach
구성 및 보관
1.2 mL Anza 12 XbaI
3 mL Anza Buffer (10X)
3 mL Anza Red Buffer (10X)
Store at -20°C.
3 mL Anza Buffer (10X)
3 mL Anza Red Buffer (10X)
Store at -20°C.
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Certificates | 증명서
Lot 번호 또는 부분 Lot 번호로 검색하십시오.
Lot 번호Certificate TypeDateCatalog Number(s)
3253170Certificate of Analysis2025년 6월 03일IVGN0128
3252877Certificate of Analysis2025년 6월 03일IVGN0126
3235838Certificate of Analysis2025년 5월 09일IVGN0128
3229012Certificate of Analysis2025년 4월 30일IVGN0126
3229004Certificate of Analysis2025년 4월 30일IVGN0126
시험 성적서 관련 5 개의 결과가 검색되었습니다.
Safety Data Sheets | 물질 안전 보건 자료
SDSScientific Resources
Product Information
자주 묻는 질문(FAQ)
All Anza restriction enzymes contain BSA.
Enzymes may freeze during shipment on dry ice. This does not affect them as they have been tested and shown to be 100% active after three freeze-thaw cycles. For 24-48 hour delivery, enzymes may be shipped on ice pack as their performance is not affected by short exposure times at 4 degrees C.
For optimal results, we highly recommend exclusively using Anza restriction enzymes in double digestion as they all have 100% buffer compatibility. If a certain enzyme is not available in Anza format, but is available in FastDigest format or as a Thermo Scientific conventional restriction enzyme, it may be possible to perform a double digestion. For specific protocol recommendations, please contact our technical support with detailed information of the desired enzymes and DNA template.
When the next step following digestion is agarose gel electrophoresis, the Anza Red buffer can be used directly. The Anza Red buffer contains the necessary loading dye and density gradient for directly loading on to the agarose gel. The red dye does not interfere with digestion and helps reduce additional pipetting.
Use the clear Anza Buffer for applications that require analysis by fluorescence excitation, as the dyes in the Anza Red Buffer may interfere with some fluorescence measurements.
Anza restriction enzymes contain a number preceding the name. This number provides the option to organize the enzymes in freezers numerically or alphabetically. More common enzymes tend to have a lower number.