Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO™ Vector also features:
• ccdB gene for positive selection (2) • EcoR I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for in vitro RNA transcription and sequencing • M13 forward and reverse primer sites for sequencing or PCR screening
Selected Zero Blunt™ TOPO™ PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주
TOP10
클로닝 방법
Blunt TOPO™
형식
Kit
제품라인
One Shot™
제품 유형
PCR Cloning Kit
프로모터
SP6
수량
50 Reactions
벡터
Blunt DNA Cloning Vectors
Unit Size
50 reactions
구성 및 보관
Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water
Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid