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카탈로그 번호 | 제품 사이즈 | 제품 정가(KRW) | 재고 정보 | 수량 | |
---|---|---|---|---|---|
M1632 | 100 reactions | 제품 정가 317,000 온라인 행사 284,000 (01-Oct-2024에 종료) 공급가 | - | ||
M1631 | 50 reactions | 제품 정가 198,000 온라인 행사 169,000 (01-Oct-2024에 종료) 공급가 | - |
Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.
Highlights
• Fast—short reaction time (typically 15–30 min)
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reaction
Applications
First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
• 20X Enzyme mix (2 x 50 μL)
• 2X RT Buffer Mix (2 x 500 μL)
Store at –15 to –25°C.
2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.