Krypton™ Fluorescent Protein Stain
Krypton™ Fluorescent Protein Stain
Thermo Scientific™

Krypton™ Fluorescent Protein Stain

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated inRead more
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Catalog NumberQuantity
46630Promo Image500 mL
Catalog number 46630
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29,340.00
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Ends: 30-Jun-2025
33,400.00
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500 mL
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Price (TWD)
29,340.00
Online offer
Ends: 30-Jun-2025
33,400.00
Save 4,060.00 (12%)
Each
Add to cart
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Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Features of Krypton Fluorescent Protein Stain:

Intense fluorescent stain—excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
Instrument-compatible—effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range—linear quantitative range spans three to four orders of magnitude Sensitive—detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
Fast—standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
Versatile—compatible with proteomics workflows involving downstream mass spectrometry
Compact—supplied as a 10X solution to save storage space; just dilute with water before use

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Product LineKrypton™
Product TypeProtein Stain
Quantity500 mL
Target MoleculeProtein
Label or DyeProprietary Mix
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
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Lot #Certificate TypeDateCatalog Number(s)
3212502Certificate of AnalysisMay 22, 202546630
ZL400545Certificate of AnalysisJan 01, 202546630
ZH398689Certificate of AnalysisSep 13, 202446630
ZC388340Certificate of AnalysisApr 17, 202446630
YK382923Certificate of AnalysisJan 25, 202446630
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Safety Data Sheets

Frequently asked questions (FAQs)

Yes. The solution will be cloudy immediately upon dilution to 1X, especially if it is cold. The cloudiness should dissipate with mixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Store Krypton Protein Stain at 4 degrees C; although it is stable enough for ambient shipping. The 10X solution is under warranty for one year from the date of shipment if handled and stored properly. The Working Solution (1X) is stable at 4 degrees C for 1-2 weeks.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Filter sets designed for Cy3 will work with the Krypton Protein Stain. Thermo Scientific scientists have used GE Healthcare Typhoon 9410 Variable Mode Imager (532 nm laser excitation; 580 BP 30 emission filter), Kodak Imager (CCD; 535 nm excitation and 600 nm emission filters), and visible light box. Sensitivity is better with lasers than with transilluminators.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Yes. HeLa cell and rat heart tissue lysates separated by 2D electrophoresis and stained with the Krypton Protein Stain provided excellent sensitivity while not over-saturating highly concentrated spots. MALDI tryptic peptide mass fingerprinting of BSA and myoglobin resulted in characteristic profiles. The proteins were destained and processed with the Thermo Scientific In-Gel Tryptic Digestion Kit (Cat. No. 89871). The lack of peak shifts indicates that the dye was not bound to the proteins after destaining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

No.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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