Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Catalog Number | Quantity |
---|---|
46630 | 500 mL |
Yes. The solution will be cloudy immediately upon dilution to 1X, especially if it is cold. The cloudiness should dissipate with mixing.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Store Krypton Protein Stain at 4 degrees C; although it is stable enough for ambient shipping. The 10X solution is under warranty for one year from the date of shipment if handled and stored properly. The Working Solution (1X) is stable at 4 degrees C for 1-2 weeks.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Filter sets designed for Cy3 will work with the Krypton Protein Stain. Thermo Scientific scientists have used GE Healthcare Typhoon 9410 Variable Mode Imager (532 nm laser excitation; 580 BP 30 emission filter), Kodak Imager (CCD; 535 nm excitation and 600 nm emission filters), and visible light box. Sensitivity is better with lasers than with transilluminators.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes. HeLa cell and rat heart tissue lysates separated by 2D electrophoresis and stained with the Krypton Protein Stain provided excellent sensitivity while not over-saturating highly concentrated spots. MALDI tryptic peptide mass fingerprinting of BSA and myoglobin resulted in characteristic profiles. The proteins were destained and processed with the Thermo Scientific In-Gel Tryptic Digestion Kit (Cat. No. 89871). The lack of peak shifts indicates that the dye was not bound to the proteins after destaining.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
No.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Share catalog number, name or link