BrdU (5-Bromo-2'-Deoxyuridine)
BrdU (5-Bromo-2'-Deoxyuridine)
Invitrogen™

BrdU (5-Bromo-2'-Deoxyuridine)

Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent深入閱讀
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產品號碼Quantity
B23151100 mg
產品號碼 B23151
價格 (TWD)
5,080.00
Online offer
Ends: 30-Sep-2025
7,240.00
您節省 2,160.00 (30%)
Each
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Quantity:
100 mg
Recurring order eligible. Learn more »
價格 (TWD)
5,080.00
Online offer
Ends: 30-Sep-2025
7,240.00
您節省 2,160.00 (30%)
Each
新增至購物車
Ask our AI about this Product
Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.
For Research Use Only. Not for use in diagnostic procedures.
規格
Label or DyeBrdU
Product TypeBrdU
Quantity100 mg
Shipping ConditionRoom Temperature
Unit SizeEach
內容物與存放
Store in freezer -5°C to -30°C and protect from light.
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常見問答集 (常見問題)

Can I combine EdU and BrdU labeling and detection on the same sample?

Yes, EdU and BrdU labeling can be combined for dual-pulse labeling of cell proliferation in cultured cells and in vivo. BrdU will be preferentially incorporated into DNA, so perform the EdU incubation first followed by the BrdU incubation. Removal of EdU from the media is not required in cultured cells when BrdU is added as the second label. Perform an alcohol fixation followed by some method of DNA denaturation as required for the BrdU detection protocol and then perform the click labeling reaction for detection of EdU followed by antibody labeling for detection of BrdU. Be sure to select a BrdU antibody that does not have cross-reactivity to EdU, such as our MoBU-1 clone (Cat. No. B35141). Many BrdU antibodies have been shown to have some amount of cross-reactivity with incorporated EdU. Here is a link (http://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/flow-cytometry-protocol/cell-proliferation/dual-pulse-labeling-of-cell-proliferation-using-edu-and-brdu-incorporation.html) to an example protocol for dual-pulse labeling using EdU and BrdU.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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證書

批號Certificate TypeDateCatalog Number(s)
3192589Certificate of AnalysisJul 13, 2025B23151
3136422Certificate of AnalysisApr 10, 2025B23151
3135758Certificate of AnalysisApr 09, 2025B23151
2984246Certificate of AnalysisOct 16, 2024B23151
913310Certificate of AnalysisOct 14, 2024B23151
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安全資料表

引用資料與參考文獻 (46)

引用資料與參考文獻
Abstract
Detection of 5-bromo-2-deoxyuridine (BrdUrd) incorporation with monoclonal anti-BrdUrd antibody after deoxyribonuclease treatment.
Authors:Takagi S, McFadden ML, Humphreys RE, Woda BA, Sairenji T
Journal:Cytometry
PubMed ID:8404370
We studied the effects of deoxyribonucleases on the detection of 5-bromo-2-deoxyuridine (BrdUrd) by anti-BrdUrd monoclonal antibodies (mAbs). After DNase I treatment, BrdUrd was detected in cells fixed on slides with the anti-BrdUrd mAbs, B44 and BMC9318. The level of detection related to the degree of DNA digestion. DNA digestion of ... More
Rac1 inhibits myogenic differentiation by preventing the complete withdrawal of myoblasts from the cell cycle.
Authors:Heller H, Gredinger E, Bengal E
Journal:J Biol Chem
PubMed ID:11489882
'The small GTPase protein Rac1 is involved in a wide range of biological processes, yet its role in cell differentiation is mostly unknown. Here we show that Rac1 activity is high in proliferating myoblasts and decreases during the differentiation process. To analyze the involvement of Rac1 in muscle differentiation, different ... More
Androgen-stimulated DNA synthesis and cytoskeletal changes in fibroblasts by a nontranscriptional receptor action.
Authors:Castoria G, Lombardi M, Barone MV, Bilancio A, Di Domenico M, Bottero D, Vitale F, Migliaccio A, Auricchio F
Journal:J Cell Biol
PubMed ID:12743104
'In NIH3T3 cells, 0.001 nM of the synthetic androgen R1881 induces and stimulates association of androgen receptor (AR) with Src and phosphatidylinositol 3-kinase (Pl3-kinase), respectively, thereby triggering S-phase entry. 10 nM R1881 stimulates Rac activity and membrane ruffling in the absence of the receptor-Src-PI3-kinase complex assembly. The antiandrogen Casodex and ... More
Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiation.
Authors:Dhawan J, Helfman DM
Journal:J Cell Sci
PubMed ID:15252113
'Cell-substratum interactions trigger key signaling pathways that modulate growth control and tissue-specific gene expression. We have previously shown that abolishing adhesive interactions by suspension culture results in G(0) arrest of myoblasts. We report that blocking intracellular transmission of adhesion-dependent signals in adherent cells mimics the absence of adhesive contacts. We ... More
Visualization of replication initiation and elongation in Drosophila.
Authors:Claycomb JM, MacAlpine DM, Evans JG, Bell SP, Orr-Weaver TL
Journal:J Cell Biol
PubMed ID:12403810
'Chorion gene amplification in the ovaries of Drosophila melanogaster is a powerful system for the study of metazoan DNA replication in vivo. Using a combination of high-resolution confocal and deconvolution microscopy and quantitative realtime PCR, we found that initiation and elongation occur during separate developmental stages, thus permitting analysis of ... More
46 total citations

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