Invitrogen™

PureLink™ Genomic DNA Mini Kit

產品號碼: K182002
Invitrogen™

PureLink™ Genomic DNA Mini Kit

產品號碼: K182002
產品號碼
K182002
包裝規格
250 preps
價格 (TWD)
價格 31,300.00
線上優惠 23,543.00
(01-Oct-2024 結束)
您的價格
供應狀況
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包裝規格
10 preps
價格 (TWD)
***
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數量
產品號碼
包裝規格
50 preps
價格 (TWD)
價格 7,650.00
線上優惠 7,190.00
(01-Oct-2024 結束)
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供應狀況
-
數量
產品號碼包裝規格價格 (TWD)供應狀況數量
K182002250 preps
價格 31,300.00
線上優惠 23,543.00
(01-Oct-2024 結束)
您的價格
-
K18200010 preps******
K18200150 preps
價格 7,650.00
線上優惠 7,190.00
(01-Oct-2024 結束)
您的價格
-
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The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).

For Research Use Only. Not for use in diagnostic procedures.

規格

Isolation Technology
Silica Spin Column
Sample Type
Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
Scale
Mini
Elution Volume
25 to 200 μL
Final Product Type
gDNA
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Test Time
Up to 15 min. after cell lysis
Yield
≤40 μg
Starting Material Amount
Bacteria: ≤109 cells
Cells: ≤106
Tissue: ≤25 mg
Mouse Tail: 1 cm
Rat Tail: 0.5 cm
Whole Blood: ≤1 mL
Yeast: ≤107 cells
FFPE: ≤8 sections of 5-15 μM thickness
Oragene™ Preserved Saliva: ≤4 mL
Blood Spots: ≤5 punches

內容物與存放

• 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature
• 45 mL PureLink Genomic Digestion Buffer; room temperature
• 50 mL PureLinkGenomic Wash Buffer 1; room temperature
• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature
• 50 mL PureLink Genomic Elution Buffer; room temperature
• 5 mL RNase A (20 mg/mL); room temperature
• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage
• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature
• 5 x 100 PureLink Collection Tubes; room temperature

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