Attune Flow Cytometer Models

Find the right Attune Flow Cytometer model for your lab

All of the Attune Flow Cytometers are compact benchtop cell-analyzers that utilize acoustic focusing technology and share common features including exceptionally fast sample flow rates, high clog resistance, and outstanding reliability. Use the comparison guide below to compare the Attune NxT Flow Cytometer with the Attune CytPix Flow Cytometer and the Attune Xenith Flow Cytometer so that you can select the right model for your research needs.

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Which Attune Flow Cytometer is right for you?

Feature
		Attune Xenith Flow Cytometer	Attune CytPix Flow Cytometer	Attune NxT Flow Cytometer
Product summary		Attune Flow Cytometer with advanced spectral capabilities including UV and NIR lasers, supporting both traditional compensation and spectral unmixing analysis.	Attune Flow Cytometer with a high-resolution brightfield camera and automated image analysis software for morphometric analysis in combination with standard fluorescence and scatter parameters.	Advanced, high throughput, acoustic focusing, benchtop flow cytometer with up to 14 color/16 parameter flow cytometry analysis.
Applications		Spectral high-parameter analyses	New users Cell and gene therapy Quality control	New users High throughput, standard parameter analysis Rare event detection
Optics (fluorescence detection)	Number of lasers	6 (fixed)	2–4 (see table below)	1–4 (see table below)
	Number of detection channels/emission filters	57 channels with wavelength-tuned photomultiplier tubes (PMTs) (51 fluorescence channels); user-changeable, keyed filters	2 scatter channels, up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
	Optical alignment	Fixed alignment with pre-aligned welded fiber; no user maintenance required
Optics (imaging)	Laser excitation	N/A	405 nm laser with <50 nanosecond pulse width	N/A
Optics (imaging)	Pulse width	N/A	<50 ns	N/A
Fluidics	Acoustic focusing	Yes	Yes	Yes
Fluidics	Custom sample flow rates	Yes	Yes	Yes
Performance (fluorescence detection)	Fluorescence sensitivity	≤30 MESF for FITC, ≤10 MESF for PE, ≤10 MESF for APC	≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
	Fluorescence resolution	rCV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)	CV <3% for the singlet peak of propidium iodide-stained chicken erythrocyte nuclei (CEN)
	Data acquisition rate	Up to 35,000 events/sec with all parameters
	Maximum electronic speed	>100,000 events/sec with all parameters	65,000 events/sec with all parameters
Performance (imaging)	Image capture rate	N/A	Up to 6,000 images/sec depending on image size and event rate	N/A
	Objective	N/A	Magnification 20x,	N/A
	Objective	N/A	Numerical aperture 0.45	N/A
	Pixel resolution	N/A	0.3 microns/pixel	N/A
	Detection limit	N/A	Visually detect 800 nm particles	N/A
Image analysis software	Models	N/A	Thermo Fisher–supplied models: leukocytes, beads; User-trained models: based on sample or particle type	N/A
	Parameters/Features	N/A	36 measured morphology parameters in 10 categories: shape features, intensity and texture features, object features, pixel features, particle interaction, particle count, similarity calculation, moment weighted, co-occurrence and system features	N/A
	Sample size range of models	N/A	5–20 µm	N/A
	Image processing speed	N/A	1,000 images/second (varies by image size and complexity)	N/A
Spectral analysis software	Spectral unmixing	Spectral unmixing with autofluorescence subtraction and live unmixing, and use of tubes and wells	N/A	N/A
Quality and regulatory	Instrument tracking	Automated daily baseline and performance test with Levey-Jennings plots
Quality and regulatory	Regulatory status	For Research Use Only
Physical	Dimensions (H x W x D) including fluid bottles	~50 x 86 x 61 cm (~19.5 x 34 x 24 in)	~49 x 58 x 43 cm (~19 x 23 x 17 in)	~40 x 58 x 43 cm (~16 x 23 x 17 in)
Physical	Biosafety hood compatibility	Yes	Yes	Yes
Computer	Software requirements	LSOne Software	Invitrogen Attune Cytometric Software
	Memory	64 GB	64 GB (4 x 16 GB) DDR4 2666 MHz UDIMM Non-ECC	32 GB
	Hard drives	2 x 8 TB SSD, 560 MB/sec; controller RAID1, integrated	2 x 8 TB SSD, 2.5-inch Samsung 870 QVO, 560 MB/s	2 x 2 TB SATA 3.0 GB/s, 8MB data burst cache; Controller RAID1, integrated
	Graphics processor	NVIDIA RTX A2000	Nvidia Quadro P2200	N/A

Laser–detector options for Attune Nxt and CytPix cytometers

Lasers	Laser configuration	Number of detection channels for included lasers					Total detection channels*	Attune CytPix	Attune NxT
Lasers	Laser configuration	Violet, 405nm	Blue, 488nm	Yellow, 561nm	Green, 532nm	Red, 637nm	Total detection channels*	Attune CytPix	Attune NxT
1	Blue	✝	4	✝	✝	✝	6	N/A	A24864
2	Blue/green	✝	3		4	✝	9	N/A	A28995
	Blue/yellow	✝	3	4		✝	9	A51842	A24861
	Blue/red	✝	4	✝	✝	3	9	A51840	A24863
	Blue/violet	4	4	✝	✝	✝	10	A51841	A24862
	Blue/violet 6	6	3	✝		✝	11	A51843	A29002
3	Blue/green/red	✝	3		4	3	12	N/A	A28997
	Blue/red/yellow	✝	3	4		3	12	A51845	A28993
	Blue/green/violet	4	3		4	✝	13	N/A	A28999
	Blue/violet/yellow	4	3	4		✝	13	A51846	A24859
	Blue/red/violet	4	4	✝	✝	3	13	A51844	A24860
	Blue/red/violet 6	6	3	✝		3	14	A51847	A29003
4	Blue/red/violet/green	4	3		4	3	16	N/A	A29001
	Blue/red/yellow/violet	4	3	4		3	16	A51848	A24858
	Blue/red/yellow/violet 6	6	2	3		3	16	A51849	A29004
^*Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. ^✝Laser upgrade available; green laser not available on Attune CytPix

Laser power specifications for Attune Flow Cytometers

Attune Xenith Flow Cytometer

Laser	Wavelength (nm)	Beam-shaping optics (BSO)* (mW)
UV	349	54
Violet	405	100
Blue	488	50
Yellow	561	50
Red	637	90
NIR	781	90

* Amount of measured usable laser power after light has gone through the beam optics and shaping filters.

Attune NxT and Attune CytPix Flow Cytometers

Laser	Wavelength (nm)	Beam-shaping optics (BSO)* (mW)	Diode power** (mW)
Violet	405	50	100
Blue	488	50	100
Green†	532	100	140
Yellow	561	50	100
Red	637	100	140

* Amount of measured usable laser power after light has gone through the beam optics and shaping filters.

** Vendor-specified theoretical maximum.

† Green laser not available on the Attune CytPix Flow Cytometer.

For Research Use Only. Not for use in diagnostic procedures.

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